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cGMP ELISA Kit is a Competitive ELISA kit for the measurement of cGMP in Plasma, Serum, Other biological fluids samples.
Alternative names=Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Other biological fluids
- Assay type
Competitive
- Range
0.00078 - 0.05 pmol/L
- Sensitivity
= 0.00047 pmol/L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
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cGMP ELISA Kit is a Competitive ELISA kit for the measurement of cGMP in Plasma, Serum, Other biological fluids samples.
Alternative names=Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Other biological fluids
- Assay type
Competitive
- Range
0.00078 - 0.05 pmol/L
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.00047 pmol/L
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
This cGMP ELISA kit (ab285321, E4717) is based on Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.
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Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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