cGMP ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of cGMP - Extracellular in Serum, Citrate plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva
- Assay type
- Competitive
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 62.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
cGMP ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of cGMP - Extracellular in Serum, Citrate plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Urine, Cell culture media, Cerebral Spinal Fluid, Saliva
- Assay type
- Competitive
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 62.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 8 | mean - | SD - | C.V. = 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 3 | mean - | SD - | C.V. = 10.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 94 - 96 % |
Sample type Citrate plasma | Average % = 100 | Range 96 - 107 % |
Sample type Heparin Plasma | Average % = 102 | Range 98 - 105 % |
Sample type Urine | Average % = 105 | Range 91 - 114 % |
Sample type Cell culture media | Average % = 93 | Range 88 - 101 % |
Sample type Cerebral Spinal Fluid | Average % = 95 | Range 91 - 102 % |
Sample type Saliva | Average % = 106 | Range 98 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
cGMP SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of cGMP in Serum, Cit Plasma, Hep Plasma, Urine, Cell culture media, Cerebrospinal Fluid, and Saliva. Quantitate cGMP with 62.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Example of cGMP standard curve in Sample Diluent NS.
OD (450 nm) values (mean +/- SD) are graphed.
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Interpolated concentration of native cGMP was measured in duplicate at different sample concentrations.
Undiluted samples are 8% Human urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2).
Sample dilutions are made in Sample Diluent NS.
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Free cGMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Undiluted samples are 25% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin.)
The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Free cGMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Undiluted samples are 25% Mouse serum, plasma (citrate), and plasma (heparin).
The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Free cGMP was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Undiluted samples are 25% Human saliva, 25% Bovine CSF, and 50% cell culture media.
The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Competitive ELISA - cGMP ELISA Kit - Extracellular (ab323638)
Serum from ten individual healthy Human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
The mean cGMP concentration in detectable samples was determined to be 851 pg/mL, with a range of 723 – 986 pg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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