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AB157690

Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit

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(4 Publications)

Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Chicken Alpha 1 Acid Glycoprotein / AGP in Chicken in Biofluids samples.

View Alternative Names

A1AG1_HUMAN, AGP, AGP 1, AGP A, Alpha 1 acid glycoprotein, Alpha-1-acid glycoprotein 1, Epididymis secretory sperm binding protein Li 153w, HEL S 153w, OMD 1, ORM, ORM1, Orosomucoid-1, alpha-1-AGP, glycoprotein, alpha-1-acid, of serum

1 Images
Functional Studies - Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB157690)
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Supplier Data

Functional Studies - Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB157690)

Representative standard curve using ab157690.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Chicken

Assay type

Sandwich (quantitative)

Sensitivity

= 1.619 ng/mL

Range

6.25 - 200 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Alpha 1 Acid Glycoprotein / AGP Chicken ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP in biological samples of chicken.

In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Product protocols

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Target data

See full target information ogchi
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 102:102431 PubMed36610106

2023

Spray-dried porcine plasma enhances feed efficiency, intestinal integrity, and immune response of broilers challenged with necrotic enteritis.

Applications

Unspecified application

Species

Unspecified reactive species

A Daneshmand,N K Sharma,T H Dao,R Barekatain,R A Swick,S-B Wu

International journal of molecular sciences 23: PubMed36233150

2022

Quantitative Proteomic Analysis Reveals Yeast Cell Wall Products Influence the Serum Proteome Composition of Broiler Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Niall Conlon,Richard A Murphy,Aoife Corrigan,Sean Doyle,Rebecca A Owens,Sheena Fagan

Poultry science 100:575-584 PubMed33518110

2021

Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine challenge.

Applications

Unspecified application

Species

Unspecified reactive species

J Herrero-Encinas,D Menoyo,M Blanch,J J Pastor,S J Rochell

Animals : an open access journal from MDPI 10: PubMed32098336

2020

Diet Supplementation with a Bioactive Pomace Extract from Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period in Broiler Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Herrero-Encinas,Marta Blanch,José J Pastor,David Menoyo
View all publications
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websiteProtocolBooklet
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