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AB157692

Chicken IgM ELISA Kit

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(5 Publications)

Chicken IgM ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Chicken IgM in Chicken in Biofluids samples.

View Alternative Names

Ig mu chain C region

1 Images
Sandwich ELISA - Chicken IgM ELISA Kit (AB157692)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IgM ELISA Kit (AB157692)

Representative standard curve using ab157692.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Chicken

Assay type

Sandwich (quantitative)

Sensitivity

= 2.73 ng/mL

Range

3.13 - 200 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IgM Chicken ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in biological samples of chicken.

In this assay the IgM present in samples reacts with the anti- IgM antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157692-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157692-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157692-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Chicken IgM ELISA Microplate", "number":"AB157692-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Chicken IgM Calibrator (lyophilized)", "number":"AB157692-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157692-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-chicken IgM antibody", "number":"AB157692-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157692-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.
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Product protocols

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Target data

See full target information
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 102:102431 PubMed36610106

2023

Spray-dried porcine plasma enhances feed efficiency, intestinal integrity, and immune response of broilers challenged with necrotic enteritis.

Applications

Unspecified application

Species

Unspecified reactive species

A Daneshmand,N K Sharma,T H Dao,R Barekatain,R A Swick,S-B Wu

Frontiers in immunology 13:892476 PubMed36032098

2022

Development and characterization of a CRISPR/Cas9-mediated knockout chicken model lacking mature B and T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Youn Lee,Hyeon Jeong Choi,Kyung Je Park,Seung Je Woo,Young Min Kim,Jae Yong Han

Frontiers in veterinary science 8:696841 PubMed34336981

2021

Performance, Serum Biochemical and Immunological Parameters, and Digestive Enzyme and Intestinal Barrier-Related Gene Expression of Broiler Chickens Fed Fermented Fava Bean By-Products as a Substitute for Conventional Feed.

Applications

Unspecified application

Species

Unspecified reactive species

Anaam E Omar,Hanan S Al-Khalaifah,Tamer Ahmed Ismail,Reda M Abd El-Aziz,Shefaa A M El-Mandrawy,Shymaa I Shalaby,Doaa Ibrahim

Journal of animal physiology and animal nutrition 106:132-138 PubMed33964044

2021

Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyi Shi,Yiran Luo,Yufa Li,Feike Zhang,Ning Liu

Frontiers in veterinary science 7:582612 PubMed33282930

2020

Effects of Phenolic-Rich Onion ( L.) Extract on the Growth Performance, Behavior, Intestinal Histology, Amino Acid Digestibility, Antioxidant Activity, and the Immune Status of Broiler Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Anaam E Omar,Hanan S Al-Khalaifah,Wafaa A M Mohamed,Heba S A Gharib,Ali Osman,Naif A Al-Gabri,Shimaa A Amer
View all publications
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