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AB157693

Chicken IgY ELISA Kit

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(4 Publications)

Chicken IgY ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Chicken IgY in Chicken in Biofluids samples.
1 Images
Sandwich ELISA - Chicken IgY ELISA Kit (AB157693)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IgY ELISA Kit (AB157693)

Representative standard curve using ab157693 IgY Chicken ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Chicken

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5305 ng/mL

Range

6.25 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IgY Chicken ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgY in chicken serum or plasma samples.

In this assay the IgY present in samples reacts with the anti-IgY antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Get results in 90 minutes with Chicken IgY ELISA Kit (ab189577) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157693-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Chicken IgY ELISA Microplate", "number":"AB157693-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157693-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157693-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157693-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157693-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Chicken IgY Calibrator (Lyophilized)", "number":"AB157693-CMP01", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-chicken IgY antibody", "number":"AB157693-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology reports (Amsterdam, Netherlands) 46:e00897 PubMed40487875

2025

Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk.

Applications

Unspecified application

Species

Unspecified reactive species

Vichununt Kerdput,Vararut Yodkamol,Mattika Sookprasong,Wongsakorn Wongwadhunyoo,Iyacoob Khunsri,Promsin Masrinoul,Trong Wisedchanwet,Naphatsamon Uthailak,Yanin Limpanont,Onrapak Reamtong,Poom Adisakwattana,Charin Thawornkuno

Poultry science 104:104699 PubMed39721261

2024

Co-evolution of the humoral immune and serotonergic systems in chickens selected for high or low blood antibody titer response to sheep red blood cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua M Lyte,Anna L Facchetti V Assumpcao,Valentina Caputi,Christopher M Ashwell,Mitiku Seyoum,Christa F Honaker,Karrie M Daniels,Mark Lyte,Paul B Siegel,Robert L Taylor

Frontiers in immunology 13:892476 PubMed36032098

2022

Development and characterization of a CRISPR/Cas9-mediated knockout chicken model lacking mature B and T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Youn Lee,Hyeon Jeong Choi,Kyung Je Park,Seung Je Woo,Young Min Kim,Jae Yong Han

Journal of animal science 96:1654-1666 PubMed29528406

2018

Supplementation of Bacillus subtilis-based probiotic reduces heat stress-related behaviors and inflammatory response in broiler chickens.

Applications

Unspecified application

Species

Unspecified reactive species

W C Wang,F F Yan,J Y Hu,O A Amen,H W Cheng
View all publications
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websiteProtocolBooklet
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