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AB273262

Chicken IL-12 p40 ELISA Kit

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Chicken IL-12 p40 ELISA Kit is a Sandwich ELISA for the measurement of Chicken IL-12 p40 in Chicken in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Chicken IL-12 p40 ELISA Kit (AB273262)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IL-12 p40 ELISA Kit (AB273262)

Chicken IL-12 p40 ELISA Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Chicken

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9 pg/mL

Range

8.19 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Chicken IL-12 p40 ELISA Kit (ab273262) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-12 p40 in serum, EDTA plasma (Citrate and Heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-12 p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-12 p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 129 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "89 - 125 %", "average": "= 102.5" }, { "sample": "Cell culture media", "range": "101 - 112 %", "average": "= 106.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273262-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273262-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-12 p40 Standard (Lyophilized)", "number":"AB273262-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273262-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-12 p40 coated Microplate (12 x 8 wells)", "number":"AB273262-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-12 p40 Antibody", "number":"AB273262-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB273262-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273262-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273262-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273262-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information IL-12 p40
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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