Chicken IL-12 p40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Chicken IL-12 p40 in Chicken in Cell culture supernatant, Serum, EDTA Plasma samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Chicken
- Range
- 8.19 - 2000 pg/mL
- Sensitivity
- = 9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL12 p40, IL12B_HUMAN, Interleukin 12 p40, Interleukin 12B, Interleukin-12 beta chain, Interleukin-12 subunit beta, NK cell stimulatory factor chain 2, NKSF, NKSF2, Natural killer cell stimulatory factor 40 kD subunit
What's included?
Recommended products
Chicken IL-12 p40 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Chicken IL-12 p40 in Chicken in Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Chicken
- Range
- 8.19 - 2000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 96 - 129 % |
Sample type EDTA Plasma | Average % = 102.5 | Range 89 - 125 % |
Sample type Cell culture media | Average % = 106.7 | Range 101 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Chicken IL-12 p40 ELISA Kit (ab273262) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-12 p40 in serum, EDTA plasma (Citrate and Heparin have not been validated yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IL-12 p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-12 p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Chicken IL-12 p40 ELISA Kit (ab273262)
Chicken IL-12 p40 ELISA Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com