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AB273263

Chicken IL-2 ELISA Kit

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(1 Publication)

Chicken IL-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Chicken IL-2 in Chicken in Cell Culture Media, Biofluids samples.

View Alternative Names

Aldesleukin, IL2_HUMAN, Interleukin-2, Involved in regulation of T cell clonal expansion, Lymphokine, OTTHUMP00000164090, POIL2, T-cell growth factor, TCGF

1 Images
Sandwich ELISA - Chicken IL-2 ELISA Kit (AB273263)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IL-2 ELISA Kit (AB273263)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Chicken

Assay type

Sandwich (quantitative)

Sensitivity

= 4.5 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Chicken IL-2 ELISA Kit (ab273263) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-2 in serum, EDTA plasma (Citrate and heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "129 - 144 %", "average": "= 139.5" }, { "sample": "Cell culture media", "range": "119 - 140 %", "average": "= 133.3" }, { "sample": "Plasma", "range": "120 - 136 %", "average": "= 130.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273263-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273263-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-2 coated Microplate (12 x 8 wells)", "number":"AB273263-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273263-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273263-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-2 Standard (Lyophilized)", "number":"AB273263-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273263-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin Concentrate", "number":"AB273263-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-2 Antibody", "number":"AB273263-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273263-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information interleukin-2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal physiology and animal nutrition 106:132-138 PubMed33964044

2021

Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyi Shi,Yiran Luo,Yufa Li,Feike Zhang,Ning Liu
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