AB273258

Chicken IL-6 ELISA Kit

Name: Chicken IL-6 ELISA Kit (ab273258) | Abcam
Brand: Abcam Limited
SKU: AB273258
Price: 825 USD
Availability: InStock

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(1 Publication)

Chicken IL-6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Chicken IL-6 in Chicken in Cell Culture Media, Biofluids samples.

View Alternative Names

Interleukin-6, ChIL-6, IL-6, IL6

1 Images

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)

sELISA

Supplier Data

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)

Chicken IL-6 ELISA Kit standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Chicken IL-6 ELISA kit (ab273258) Standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Chicken

Assay type

Sandwich (quantitative)

Sensitivity

= 0.085 ng/mL

Range

0.082 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Chicken IL-6 ELISA Kit (ab273258) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-6 in serum, EDTA plasma (Citrate and heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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Recovery

[ { "sample": "Serum", "range": "71 - 114 %", "average": "= 96.57" }, { "sample": "Cell culture media", "range": "116 - 140 %", "average": "= 129.7" }, { "sample": "Plasma", "range": "72 - 80 %", "average": "= 76.16" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273258-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273258-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-6 Antibody", "number":"AB273258-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273258-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273258-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273258-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273258-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-6 coated Microplate (12 x 8 wells)", "number":"AB273258-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-6 Standard (Lyophilized)", "number":"AB273258-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273258-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

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Product protocols

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Target data

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.

See full target information IL6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal physiology and animal nutrition 106:132-138 PubMed33964044

2021

Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyi Shi,Yiran Luo,Yufa Li,Feike Zhang,Ning Liu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Chicken IL-6 ELISA Kit

Sandwich ELISA - Chicken IL-6 ELISA Kit (AB273258)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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