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Chicken SAA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Chicken SAA in Chicken in Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Chicken

Range

0.375 - 24 ng/mL

Assay time

1h 30m

Sensitivity

= 0.094 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
100X HRP Conjugated Enzyme Antibody
1 x 150 µL
1X Diluent Concentration
1 x 60 mL
20X Wash Buffer Concentrate
1 x 50 mL
Anti-Chicken SAA ELISA Microplate
1 x 1 Unit
Chicken SAA Calibrator (Lyophilized)
1 x 1 Vial
Chromogen Substrate Solution
1 x 12 mL
Stop Solution
1 x 12 mL

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Chicken SAA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Chicken SAA in Chicken in Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Chicken

Range

0.375 - 24 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.094 ng/mL

General recovery

>= 85%

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

>= 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in chicken plasma and serum.

In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Supplementary info

Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Activity summary

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

Associated diseases and disorders

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Chicken SAA ELISA Kit (ab205081), expandable thumbnail

    Sandwich ELISA - Chicken SAA ELISA Kit (ab205081)

    Representative standard curve using ab205081 Chicken SAA ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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