AB240996
CHO HCP ELISA kit (Host cell protein)
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CHO HCP ELISA kit (Host cell protein) is a sandwich ELISA used to quantify CHO Host cell protein contamination.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Sensitive measurement of CHO host cell protein, detecting as little as 10 ng/ml
- Results in 3 hours
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Sensitive measurement of CHO host cell protein, detecting as little as 10 ng/ml
- Results in 3 hours
1 Images
- sELISA
Supplier Data
Sandwich ELISA - CHO HCP ELISA kit (Host cell protein) (AB240996)
Example of CHO Host Cell Protein standard curve.
The standard curve was prepared as described in Section 10.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
CHO HCP ELISA kit (Host cell protein) ab240996 is a sandwich ELISA to measure CHO HCP (Host cell protein) in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems with a sensitivity of 10 ng/mL.
How the assay works:
The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological samples. Following an incubation to allow capture of the CHO protein by the antibodies on the plate, a second anti-CHO HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured CHO proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
How the assay works:
The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological samples. Following an incubation to allow capture of the CHO protein by the antibodies on the plate, a second anti-CHO HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured CHO proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
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