Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Cleaved Tau (Human Asp738/Mouse Asp713) in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Human
- Range
- 0.313 - 10 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.075 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
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Recommended products
Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Cleaved Tau (Human Asp738/Mouse Asp713) in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Human
- Range
- 0.313 - 10 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.075 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 88 | Range 83 - 92 % |
Sample type Serum | Average % = 105 | Range 103 - 109 % |
Sample type EDTA Plasma | Average % = 93 | Range 90 - 97 % |
Sample type Tissue Extracts | Average % = 92 | Range 93 - 97 % |
Sample type Heparin Plasma | Average % = 107 | Range 103 - 113 % |
Sample type Citrate plasma | Average % = 95 | Range 91 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cleaved Tau (Human Asp738/Mouse Asp713) protein in cell culture supernatant, cell lysate, edta plasma, serum, cit plasma, hep plasma, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Cleaved Tau (Human Asp738/Mouse Asp713) with 0.075 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tau promotes microtubule assembly and stability; it might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Axonal polarity is predetermined by Tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Tau is expressed predominantly in neurons. Human Tau is expressed at least as 9 isoforms. The long PNS-tau isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau is phosphorylated at various serine and threonine residues. Tau phosphorylation impairs the Tau ability to bind microtubules and leads to microtubule depolymerization. Hyperphosphorylated Tau is the major component of paired helical filaments, the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Tau truncation can affect Tau pathologic characteristics, including its ability to acquire AD-related conformations and to assemble into filaments. Amyloid-beta protein can trigger caspase activation and cellular apoptosis. Several activated caspases, including caspase-3, caspase-7, and caspase-8, can cleave Tau at a highly conserved sequence present in all isoforms generating Cleaved Tau with …SSTGSIDMVD sequence at the carboxy terminus. This carboxy terminal Asp corresponds to, for example, Asp738 of human canonical PNS isoform, Asp421 of human Tau-F isoform, or Asp713 of mouse canonical PNS isoform. The cleavage of Tau at this site is an important inducer of Tau polymerization in AD. This kit is designed to detect all Tau isoforms only when cleaved at the above described site.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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5 product images
Sandwich ELISA - Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557)
Example of human and mouse Cleaved Tau standard curve in Sample Diluent NS.
The Cleaved Tau standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557)
Interpolated concentrations of native Cleaved Tau in mouse brain supernatant sample.
The concentrations of Cleaved Tau were measured in duplicates, interpolated from the Cleaved Tau standard curves and corrected for sample dilution. Undiluted sample is as follows: mouse brain supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cleaved Tau concentration was determined to be 4.68 ng/mL in neat brain supernatant.
Sandwich ELISA - Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557)
Interpolated concentrations of native Cleaved Tau in mouse brain tissue, 1 µM staurosporine treated and mock treated Neuro-2a cell extracts based on 250 μg/mL, 250 µg/mL and 500 µg/mL extract loads.
The concentrations of Cleaved Tau were measured in duplicate and interpolated from the Cleaved Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cleaved Tau concentration was determined to be 2.19 ng/mL in mouse brain extract, 6.27 ng/mL in staurosporine treated Neuro-2a extract and 0.426 ng/mL in mock treated Neuro-2a extract.
Sandwich ELISA - Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557)
Interpolated concentrations of native Cleaved Tau in 2 and 4 hours 1 µM staurosporine treated SH-SY5Y and mock treated cell extract samples based on 1000 μg/mL, 250 µg/mL and 1000 µg/mL extract loads.
The concentrations of Cleaved Tau were measured in duplicate and interpolated from the Cleaved Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cleaved Tau concentration was determined to be 7.93 ng/ mL in 2 hours staurosporine treated SH-SY5Y extract, 5.80 in 4 hours staurosporine treated SH-SY5Y extract and 2.42 in mock treated SH-SY5Y extract.
Sandwich ELISA - Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) (ab269557)
Comparison of staurosporine treated and mock treated Neuro-2a and SH-SY5Y cell extracts.
SH-SY5Y cells were cultured in the presence of 1 μM staurosporine for 2 hours and 4 hours, or in the presence of staurosporine solvent (mock) for 4 hours. Neuro-2a cells were cultured in the presence of 1 μM staurosporine for 4 hours, or in the presence of staurosporine solvent (mock) for 4 hours. The concentrations of Cleaved Tau were measured in three different dilutions of the cell extract samples in duplicates and interpolated from the Cleaved Tau standard curve. The interpolated dilution factor corrected values are plotted in ng of Cleaved Tau per mg of extract (mean +/- SD, n=3). The mean Cleaved Tau concentration was determined to be 0.85 ng/mg in Neuro-2a (mock), 24.6 ng/mg in Neuro-2a (4-hours STS), 2.37 ng/mg in SH-SY5Y (mock), 7.49 ng/mg in SH-SY5Y (2-hours STS), 22.2 ng/mg in SH-SY5Y (4-hours STS) cell extracts.
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