Complex I Rodent Profiling ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Complex I Rodent Profiling in Mouse, Rat, Human in Tissue Extracts samples.
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- Folia neuropathologica 59:50-662021Inhibition of ASC enhances the protective role of salvianolic acid A in traumatic brain injury via inhibition of inflammation and recovery of mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Chao Xu,Kunhu Zhang,Jianjun Shi,Zhenyang Liu,Xinxing Wang,Min Guo,Jiannan Lv,Xinmin DingPubMed 33969677
- Cell reports 31:1075062020Cell Metabolic Alterations due to Mcph1 Mutation in Microcephaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathalie Journiac,Javier Gilabert-Juan,Sara Cipriani,Paule Benit,Xiaoqian Liu,Sandrine Jacquier,Valérie Faivre,Andrée Delahaye-Duriez,Zsolt Csaba,Tristan Hourcade,Eliza Melinte,Sophie Lebon,Céline Violle-Poirsier,Jean-François Oury,Homa Adle-Biassette,Zhao-Qi Wang,Shyamala Mani,Pierre Rustin,Pierre Gressens,Jeannette NardelliPubMed 32294449
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (qualitative)
- Reactive species
- Mouse, Rat, Human
- Range
- 4 - 500 µg/mL
- Sensitivity
- = 0.4 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Complex I Rodent Profiling ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Complex I Rodent Profiling in Mouse, Rat, Human in Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (qualitative)
- Reactive species
- Mouse, Rat, Human
- Range
- 4 - 500 µg/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.4 µg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Rat Heart | n 8 | C.V. 2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Rat Heart | n 3 | C.V. 6.14 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ab136809 NADH dehydrogenase (Complex I) rodent profiling kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of NADH dehydrogenase levels or profile in tissue lysates. The assay employs an NADH dehydrogenase specific antibody coated onto microplate well plate strips. Samples are pipetted into the wells and NADH dehydrogenase present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-NADH dehydrogenase detector antibody is added. After washing away unbound detector antibody, biotin affinity HRP conjugated streptavidin is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of NADH dehydrogenase (Complex I) bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Get results in 90 minutes with Human NADH dehydrogenase ELISA Kit (Complex I) (Human NADH dehydrogenase ELISA Kit (Complex I) ab178011) from our SimpleStep ELISA® range.
NADH dehydrogenase (Complex I) is the first enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. NADH dehydrogenase is a large protein complex of 950,000 MW made up of 45-46 different subunits. Seven of the subunits of the complex are encoded on mitochondrial DNA (mtDNA), the remaining subunits are nuclear encoded, made in the cytosol and translocated into the organelle for assembly at the inner membrane. The enzyme complex catalyses electron entry from NADH via a flavin (FMN) and several non-heme iron centers. Mutations in mtDNA, or nuclear DNA genes encoding NADH dehydrogenase subunits or assembly factors are a common cause of genetic OXPHOS defects. Mutations or loss of mtDNA may cause enzymatic dysfunction by disrupting enzyme assembly or alternatively by specifically affecting enzymatic activity with no effect on enzyme assembly.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex I also known as NADH dehydrogenase or NADH:ubiquinone oxidoreductase is a large enzyme complex with a molecular mass of approximately 1000 kDa. It is expressed in the inner mitochondrial membrane of eukaryotic cells. As the first enzyme in the mitochondrial respiratory chain Complex I plays a critical mechanical role in cellular respiration. It transfers electrons from NADH to ubiquinone coupled with the translocation of protons across the inner mitochondrial membrane contributing to the generation of a proton gradient used to produce ATP.
- Biological function summary
Complex I acts as an integral component of the mitochondrial respiratory chain which is a series of protein complexes involved in cellular energy production. As part of this complex system Complex I is essential for effective oxidative phosphorylation. Its activity is assessed using protein activity assays including immunocapture or complex activity assays and microplate assays. Complex I activity influences the overall efficiency of ATP production affecting energy-dependent cellular processes.
- Pathways
Complex I functions within the electron transport chain one of the major pathways in cellular respiration. This pathway is vital for ATP synthesis providing the energy currency required by cells. Complex I works closely with other electron transport chain complexes such as Complex II (succinate dehydrogenase complex) and Complex III (cytochrome c reductase) to drive oxidation-reduction reactions and maintain cellular metabolism.
- Associated diseases and disorders
Defects in Complex I are linked with mitochondrial diseases and neurodegenerative disorders such as Leigh syndrome and Parkinson's disease. Mutations in Complex I subunits disrupt normal electron transport and ATP production leading to increased oxidative stress and neuronal cell damage. Other mitochondrial proteins such as cytochrome c oxidase are also implicated in these conditions emphasizing the interconnected nature of mitochondrial dysfunction in disease progression.
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ELISA - Complex I Rodent Profiling ELISA Kit (ab136809)
TYPICAL STANDARD CURVE - For demonstration only. Example of positive control sample standard curve. A dilution series of mouse heart homogenate (MHH) extract in Incubation Buffer in the working range of the assay.
ELISA - Complex I Rodent Profiling ELISA Kit (ab136809)
TYPICAL STANDARD CURVE - For demonstration only. Example of positive control sample standard curve. A dilution series of rat heart homogenate (RHH) extract in Incubation Buffer in the working range of the assay.
Functional Studies - Complex I Rodent Profiling ELISA Kit (ab136809)
Complex I Rodent Profiling ELISA Kit. Components requirement and immune assay specificity were determined by skipping one critical component or replacing with unrelated antibodies. The sample was rat heart homogenate (RHH) at the concentration of 500 µg/mL.
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