Corticosterone ELISA kit

Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, and saliva samples.

A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

Please note the concentrations of the kit components are lot-specific and the end user should always refer to the vial label

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.