Cortisol ELISA kit is a competitive ELISA designed to quantify total human cortisol with a sensitivity of 2.44 ng/mL.
- Wide dynamic range – quantifies 10–500 ng/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, heparin), and urine
- Assay platform – microplate (12 x 8 well strips) for easy setup and reliable results
- Cited in over 15 publications
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- Scientific reports 13:155082023A 3D adrenocortical carcinoma tumor platform for preclinical modeling of drug response and matrix metalloproteinase activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya H Dedhia,Hemamylammal Sivakumar,Marco A Rodriguez,Kylie G Nairon,Joshua M Zent,Xuguang Zheng,Katie Jones,Liudmila V Popova,Jennifer L Leight,Aleksander SkardalPubMed 37726363
- BMC musculoskeletal disorders 24:802023Sex-specific effects of calving season on joint health and biomarkers in Montana ranchers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew A Thompson,Stephen A Martin,Brady D Hislop,Roubie Younkin,Tara M Andrews,Kaleena Miller,Ronald K June,Erik S AdamsPubMed 36717802
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22041712022MicroSweat: A Wearable Microfluidic Patch for Noninvasive and Reliable Sweat Collection Enables Human Stress Monitoring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaghayegh Shajari,Razieh Salahandish,Azam Zare,Mohsen Hassani,Shirin Moossavi,Emily Munro,Ruba Rashid,David Rosenegger,Jaideep S Bains,Amir Sanati NezhadPubMed 36461733
- PloS one 17:e02700932022Effectiveness of short-term isothermic-heat acclimation (4 days) on physical performance in moderately trained males.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJake Shaw,Cory Walkington,Edward Cole,Damien O Gleadall-Siddall,Rachel Burke,James Bray,Andrew J Simpson,Rebecca V Vince,Andrew T GarrettPubMed 36395344
- Frontiers in oncology 12:9551842022Impact of integrated yoga therapy on cognitive impairment and cardiac dysfunction in relation to quality of life in breast cancer patients undergoing chemotherapy: Study protocol for a two-arm randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGanagarajan Inbaraj,Talakad N Sathyaprabha,Kaviraja Udupa,Amritanshu Ram,Shekar Patil,Jamuna Rajeswaran,Krishna K Nandakumar,Spoorthi Belur,Arman Deep Singh,Parthipulli Vasuki Prathyusha,Sapna K Bayari,Rao M RaghavendraPubMed 36185291
- Frontiers in physiology 13:8368102022Dietary Inclusion of Hydrolyzed Yeasts Modulates Physiological Responses in Plasma and Immune Organs of Atlantic Salmon () Parr Exposed to Acute Hypoxia Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesByron Morales-Lange,Brankica Djordjevic,Ashwath Gaudhaman,Charles McLean Press,Jake Olson,Liv Torunn Mydland,Luis Mercado,Mónica Imarai,Mathieu Castex,Margareth ØverlandPubMed 35418880
- Brain and behavior 11:e23002021Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxiu Chang,Li Zhang,Huiyu Dai,Ling SunPubMed 34333865
- International journal of molecular sciences 22:2021Comparison of Circulating Markers and Mucosal Immune Parameters from Skin and Distal Intestine of Atlantic Salmon in Two Models of Acute Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrankica Djordjevic et. al.PubMed 33494146
- Computational and structural biotechnology journal 18:3678-36912020Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWaqas Khaliq et. al.PubMed 33304464
- International journal of molecular sciences 20:2019Incomplete Spinal Cord Injury Reverses the Level-Dependence of Spinal Cord Injury Immune Deficiency Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Hong et. al.PubMed 31374824
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 10 - 500 ng/mL
- Sensitivity
- = 2.44 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Cortisol ELISA kit is a competitive ELISA designed to quantify total human cortisol with a sensitivity of 2.44 ng/mL.
- Wide dynamic range – quantifies 10–500 ng/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, heparin), and urine
- Assay platform – microplate (12 x 8 well strips) for easy setup and reliable results
- Cited in over 15 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Heparin Plasma, Serum, Urine
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 10 - 500 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 2.44 ng/mL
- General recovery
- 103.56%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. <= 9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 30 | mean - | SD - | C.V. <= 9.8 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Cortisol ELISA kit ab108665 is a competitive ELISA kit to measure total human cortisol in serum and plasma with a sensitivity of 2.44 ng/mL.
How the assay works
Cortisol ELISA kit ab108665 is a competitive ELISA. There are 2 types of competitive ELISAs:
- antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
- antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Cortisol ELISA kit ab108665 protocol summary
- Add standards or samples to the wells, incubate 2 hrs
- Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
- Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
- Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
- Add 50 µl of Stop Solution per well. Read at 450 nm immediately
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Competitive ELISA - Cortisol ELISA Kit (ab108665)
Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
Competitive ELISA - Cortisol ELISA Kit (ab108665)
Representative Standard Curve using ab108665
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- Visit the general protocols
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