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AB108665

Cortisol ELISA Kit

5

(4 Reviews)

|

(21 Publications)

Cortisol ELISA kit is a competitive ELISA designed to quantify total human cortisol with a sensitivity of 2.44 ng/mL.

- Wide dynamic range – quantifies 10–500 ng/mL
- Compatible with multiple sample types – works with serum, plasma (citrate, heparin), and urine
- Assay platform – microplate (12 x 8 well strips) for easy setup and reliable results
- Cited in over 15 publications

2 Images
Competitive ELISA - Cortisol ELISA Kit (AB108665)
  • cELISA

Supplier Data

Competitive ELISA - Cortisol ELISA Kit (AB108665)

Representative Standard Curve using ab108665

Competitive ELISA - Cortisol ELISA Kit (AB108665)
  • cELISA

Lab

Competitive ELISA - Cortisol ELISA Kit (AB108665)

Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum, Urine

Reacts with

Human

Assay type

Competitive

Sensitivity

= 2.44 ng/mL

Range

10 - 500 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Cortisol ELISA kit ab108665 is a competitive ELISA kit to measure total human cortisol in serum and plasma with a sensitivity of 2.44 ng/mL.

How the assay works

Cortisol ELISA kit ab108665 is a competitive ELISA. There are 2 types of competitive ELISAs:

  • antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
  • antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 9" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 9.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108665-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108665-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Control", "number":"AB108665-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Washing Solution", "number":"AB108665-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108665-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 2 – 50 ng/mL", "number":"AB108665-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108665-CMP09", "productcode":"" }, { "size":"1 x 21 mL", "name":"Cortisol-HRP conjugate", "number":"AB108665-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 3 – 150 ng/mL", "number":"AB108665-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 0 – 0 ng/mL", "number":"AB108665-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 1 – 10 ng/mL", "number":"AB108665-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 4 – 500 ng/mL", "number":"AB108665-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Cortisol
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:3198-3206 PubMed40384998

2025

The effect of an intravenous analgesic pump with esketamine on postoperative pain and postpartum depression in women with cesarean section.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Xiao,Baoyi Su,Zhuoyu You,Hui Lu,Tuanming Huang

CNS neuroscience & therapeutics 31:e70153 PubMed39957482

2025

Study on the Mechanisms of Ischemic Stroke Impacting Sleep Homeostasis and Circadian Rhythms in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Ting Chu,Chen Sun,Yong-Hui Zheng,Wen-Ying Gao,Lin-Lin Zhao,Jing-Yu Zhang

Heliyon 10:e39534 PubMed39498052

2024

Blockade of 11β-hydroxysteroid dehydrogenase type 1 ameliorates metabolic dysfunction-associated steatotic liver disease and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hwan Ma,Guo-Yan Sui,Jeong-Su Park,Feng Wang,Yuanqiang Ma,Dong-Su Shin,Nodir Rustamov,Jun Sung Jang,Soo Im Chang,Jin Lee,Yoon Seok Roh

Journal of multidisciplinary healthcare 17:4549-4557 PubMed39371400

2024

Association Between Dry Eye Disease with Anxiety and Depression Among Medical Sciences Students in Qassim Region: Cortisol Levels in Tears as a Stress Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Hanan Awad Alkozi,Hanin Abdullah Alhudhayf,Norah Mohammed Abdulrahman Alawad

Scientific reports 13:15508 PubMed37726363

2023

A 3D adrenocortical carcinoma tumor platform for preclinical modeling of drug response and matrix metalloproteinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priya H Dedhia,Hemamylammal Sivakumar,Marco A Rodriguez,Kylie G Nairon,Joshua M Zent,Xuguang Zheng,Katie Jones,Liudmila V Popova,Jennifer L Leight,Aleksander Skardal

BMC musculoskeletal disorders 24:80 PubMed36717802

2023

Sex-specific effects of calving season on joint health and biomarkers in Montana ranchers.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew A Thompson,Stephen A Martin,Brady D Hislop,Roubie Younkin,Tara M Andrews,Kaleena Miller,Ronald K June,Erik S Adams

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2204171 PubMed36461733

2022

MicroSweat: A Wearable Microfluidic Patch for Noninvasive and Reliable Sweat Collection Enables Human Stress Monitoring.

Applications

Unspecified application

Species

Unspecified reactive species

Shaghayegh Shajari,Razieh Salahandish,Azam Zare,Mohsen Hassani,Shirin Moossavi,Emily Munro,Ruba Rashid,David Rosenegger,Jaideep S Bains,Amir Sanati Nezhad

PloS one 17:e0270093 PubMed36395344

2022

Effectiveness of short-term isothermic-heat acclimation (4 days) on physical performance in moderately trained males.

Applications

Unspecified application

Species

Unspecified reactive species

Jake Shaw,Cory Walkington,Edward Cole,Damien O Gleadall-Siddall,Rachel Burke,James Bray,Andrew J Simpson,Rebecca V Vince,Andrew T Garrett

Frontiers in oncology 12:955184 PubMed36185291

2022

Impact of integrated yoga therapy on cognitive impairment and cardiac dysfunction in relation to quality of life in breast cancer patients undergoing chemotherapy: Study protocol for a two-arm randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Ganagarajan Inbaraj,Talakad N Sathyaprabha,Kaviraja Udupa,Amritanshu Ram,Shekar Patil,Jamuna Rajeswaran,Krishna K Nandakumar,Spoorthi Belur,Arman Deep Singh,Parthipulli Vasuki Prathyusha,Sapna K Bayari,Rao M Raghavendra

Frontiers in physiology 13:836810 PubMed35418880

2022

Dietary Inclusion of Hydrolyzed Yeasts Modulates Physiological Responses in Plasma and Immune Organs of Atlantic Salmon () Parr Exposed to Acute Hypoxia Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Byron Morales-Lange,Brankica Djordjevic,Ashwath Gaudhaman,Charles McLean Press,Jake Olson,Liv Torunn Mydland,Luis Mercado,Mónica Imarai,Mathieu Castex,Margareth Øverland
View all publications
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