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AB108674

Cortisol ELISA Kit

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(1 Publication)

Cortisol ELISA Kit is a competitive ELISA designed to quantify Cortisol with a sensitivity of 2.95  ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 10 - 500 ng/mL
1 Images
Competitive ELISA - Cortisol ELISA Kit (AB108674)
  • cELISA

Unknown

Competitive ELISA - Cortisol ELISA Kit (AB108674)

Representative Standard Curve using ab108674

Key facts

Detection method

Colorimetric

Sample types

Urine

Reacts with

Human

Assay type

Competitive

Sensitivity

= 2.95 ng/mL

Range

10 - 500 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.

A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 7.2" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 6.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108674-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108674-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Cortisol IgG Coated Microplate (12 x 8 wells)", "number":"AB108674-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foils", "number":"AB108674-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108674-CMP13", "productcode":"" }, { "size":"1 x 33 mL", "name":"Cortisol-HRP Conjugate", "number":"AB108674-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108674-CMP14", "productcode":"" }, { "size":"1 x 4 mL", "name":"Cortisol Standard 0 - 0 ng/mL", "number":"AB108674-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 4 – 200 ng/mL", "number":"AB108674-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Wash Solution", "number":"AB108674-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 2 - 5 ng/mL", "number":"AB108674-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Low control", "number":"AB108674-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 1 - 1 ng/mL", "number":"AB108674-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"High control", "number":"AB108674-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 3 - 30 ng/mL", "number":"AB108674-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Cortisol
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, and immunity 80:725-741 PubMed31100372

2019

Molecular alterations induced by Yersinia pestis, dengue virus and Staphylococcal enterotoxin B under severe stress.

Applications

Unspecified application

Species

Unspecified reactive species

Seid Muhie,Ross Campbell,Aarti Gautam,Rasha Hammamieh,Christiano Cummings,Marti Jett
View all publications
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websiteProtocolBooklet
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