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AB154996

Cortisol ELISA Kit

5

(2 Reviews)

|

(11 Publications)

Cortisol ELISA Kit is a competitive ELISA used to quantify cortisol with a sensitivity of 0.12 ng/mL.

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide measuring range: 0.5 - 100 ng/mL
1 Images
Competitive ELISA - Cortisol ELISA Kit (AB154996)
  • cELISA

Supplier Data

Competitive ELISA - Cortisol ELISA Kit (AB154996)

Representative Standard Curve using ab154996.

Key facts

Detection method

Colorimetric

Sample types

Saliva

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.12 ng/mL

Range

0.5 - 100 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.

A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Saliva", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 8.3" }, { "reproducibilityType": "Intra", "sample": "Saliva", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab154996-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Incubation Buffer", "number":"AB154996-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol-HRP Conjugate (Concentrated)", "number":"AB154996-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control B", "number":"AB154996-CMP16", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control A", "number":"AB154996-CMP15", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB154996-CMP14", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Wash Solution", "number":"AB154996-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 2 – 1 ng/mL", "number":"AB154996-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 5 – 20 ng/mL", "number":"AB154996-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 1 - 0.5 ng/mL", "number":"AB154996-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 6 – 100 ng/mL", "number":"AB154996-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 3 – 5 ng/mL", "number":"AB154996-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 4 – 10 ng/mL", "number":"AB154996-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cortisol Standard 0 – 0 ng/mL", "number":"AB154996-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Cortisol IgG Coated Microplate (12 x 8 wells)", "number":"AB154996-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB154996-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Foil Cover", "number":"AB154996-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB154996-CMP17", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Cortisol
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Education sciences 14: PubMed39741652

2025

From Surviving to Thriving: A Trauma-Informed Yoga Intervention for Adolescents and Educators in Rural Montana.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Davis,Brandon G Scott,Greta M Linse,Rebecca Buchanan

Education sciences 13: PubMed38116307

2023

"Following the Breath:" A Trauma-Informed Intervention for Educator Wellness in Rural Montana.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Davis,Alexandra Aylward,Brandon G Scott,Jonathan Jacobs

Acta paediatrica (Oslo, Norway : 1992) 112:1011-1018 PubMed36740937

2023

Effects of prolonged sitting and physical activity breaks on measures of arterial stiffness and cortisol in adolescents.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Fernström,Emerald G Heiland,Karin Kjellenberg,Marjan Ponten,Olga Tarassova,Gisela Nyberg,Björg Helgadottir,Maria M Ekblom,Örjan Ekblom

Journal of evidence-based integrative medicine 28:2515690X221150527 PubMed36659818

2023

Aromatherapy Reduces Anxiety in Unexperienced Patients Hospitalized for Interventional Neuroradiology Procedures: A Randomized Control Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Jetsada Sriboonlert,Waranon Munkong,Sunantha Rintawut,Soodjai Paladkhua,Ratchalita Suwongsa,Woranan Kirisattayakul

Journal of child & adolescent trauma :1-14 PubMed36471891

2022

Mitigating Rural Adolescent Trauma: Remote Delivery of a Trauma-Informed Yoga Intervention During COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Davis,Alexandra Aylward

Educational studies 58:530-559 PubMed36654845

2022

Trauma-Informed Yoga: Investigating an Intervention for Mitigating Adverse Childhood Experiences in Rural Contexts.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Davis,Alexandra Aylward,Rebecca Buchanan

Trials 23:22 PubMed34991692

2022

ABBaH teens: Activity Breaks for Brain Health in adolescents: study protocol for a randomized crossover trial.

Applications

Unspecified application

Species

Unspecified reactive species

Emerald G Heiland,Karin Kjellenberg,Olga Tarassova,Maria Fernström,Gisela Nyberg,Maria M Ekblom,Björg Helgadottir,Örjan Ekblom

Frontiers in human neuroscience 15:719509 PubMed34602995

2021

Frequent, Short Physical Activity Breaks Reduce Prefrontal Cortex Activation but Preserve Working Memory in Middle-Aged Adults: ABBaH Study.

Applications

Unspecified application

Species

Unspecified reactive species

Emerald G Heiland,Olga Tarassova,Maria Fernström,Coralie English,Örjan Ekblom,Maria M Ekblom

International journal of environmental research and public health 18: PubMed34071628

2021

Hormonal Differences in Intimate Partner Violence Perpetrators When They Cope with Acute Stress: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ángel Romero-Martínez,Mari-Carmen Blanco-Gandía,Marta Rodriguez-Arias,Marisol Lila,Luis Moya-Albiol

Frontiers in human neuroscience 14:273 PubMed32760263

2020

ABBaH: Activity Breaks for Brain Health. A Protocol for a Randomized Crossover Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Emerald G Heiland,Örjan Ekblom,Olga Tarassova,Maria Fernström,Coralie English,Maria M Ekblom
View all publications
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