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Cortisol ELISA Kit ab154996 is used to quantify Cortisol with a sensitivity of 2.9 pg/ml.

Competitive ELISA Kit


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Competitive ELISA - Cortisol ELISA Kit (AB154996), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Saliva
Assay type
Competitive
Reactive species
Human
Range
0.5 - 100 ng/mL
Sensitivity
= 0.12 ng/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Select an associated product type

2 products for Alternative Product

Target data

What's included?

1 x 96 Tests
Components
10X Wash Solution
1 x 50 mL
Anti-Cortisol IgG Coated Microplate (12 x 8 wells)
1 x 1 Unit
Control A
1 x 1 mL
Control B
1 x 1 mL
Cortisol Standard 0 – 0 ng/mL
1 x 1 mL
Cortisol Standard 1 - 0.5 ng/mL
1 x 1 mL
Cortisol Standard 2 – 1 ng/mL
1 x 1 mL
Cortisol Standard 3 – 5 ng/mL
1 x 1 mL
Cortisol Standard 4 – 10 ng/mL
1 x 1 mL
Cortisol Standard 5 – 20 ng/mL
1 x 1 mL
Cortisol Standard 6 – 100 ng/mL
1 x 1 mL
Cortisol-HRP Conjugate (Concentrated)
1 x 1 mL
Foil Cover
1 x 1 Unit
Incubation Buffer
1 x 30 mL
Stop Solution
1 x 15 mL
Strip holder
1 x 1 Unit
TMB Substrate Solution
1 x 15 mL

Recommended products

Cortisol ELISA Kit ab154996 is used to quantify Cortisol with a sensitivity of 2.9 pg/ml.

Competitive ELISA Kit

Key facts

Detection method
Colorimetric
Sample types
Saliva
Assay type
Competitive
Reactive species
Human
Range
0.5 - 100 ng/mL
Assay Platform
Microplate
Sensitivity
= 0.12 ng/mL
General recovery
95.42%

Precision

Intra assay

Sample
Saliva
n
60
mean
-
SD
-
C.V.
<= 10

Inter assay

Sample
Saliva
n
30
mean
-
SD
-
C.V.
<= 8.3

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Abcam's Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.

A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Competitive ELISA - Cortisol ELISA Kit (ab154996), expandable thumbnail

    Competitive ELISA - Cortisol ELISA Kit (ab154996)

    Representative Standard Curve using ab154996.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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