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AB324664

Cotinine ELISA Kit - Intracellular

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Cotinine ELISA Kit - Intracellular is a Competitive ELISA for the measurement of Cotinine - Intracellular in in Cell/Tissue Extracts samples.
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Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)

Example of ​Cotinine​ standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)

Free ​Cotinine​ was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 500 µg/mL A549 cell extract, HeLa cell extract, Jurkat cell extract, and Human lung extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell Extract, Tissue Extracts

Assay type

Competitive

Sensitivity

= 18.804 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Cotinine ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Cotinine with a sensitivity of 18.804pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric competitive ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders

-A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" }, { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "95 - 101 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "92 - 104 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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