COVID-19 N-Protein Human IgA ELISA Kit is a Semi-quantitative ELISA kit for the measurement of COVID-19 N-Protein Human IgA in Plasma, Serum samples.
| Application | Reactivity | Dilution info | Notes |
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Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
COVID-19 N-Protein Human IgA ELISA Kit is a Semi-quantitative ELISA kit for the measurement of COVID-19 N-Protein Human IgA in Plasma, Serum samples.
COVID-19 N-Protein Human IgA ELISA Kit (ab276183) is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SARS-CoV-2 N protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRP human IgA antibody virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control is from an inactivated serum sample which contains SARS-COV-2 N protein human IgA antibody. We do not know the exact amount of SARS-COV-2 N protein human IgA antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.
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COVID-19 N-Protein also known as the nucleocapsid protein of SARS-CoV-2 plays a fundamental role in the virus's life cycle. Mechanically it binds to the viral RNA genome forming a ribonucleoprotein complex that is critical for viral replication. N-Protein's approximate molecular mass is around 46 kDa. It is expressed in the cytoplasm of infected cells and is highly abundant making it an important target for diagnostic assays like the COVID-19 ELISA test.
The N-Protein functions as a structural component of the coronavirus. It facilitates the encapsidation of the viral genome and forms part of the viral replication-transcription complexes. The N-Protein interacts with other viral proteins such as the membrane (M) protein indicating its involvement in viral assembly and release. It influences the host cell's response to infection by modulating various cellular processes.
The COVID-19 N-Protein participates in the replication and transcription pathway of SARS-CoV-2. It engages with RNA polymerase and NSP3 to support efficient viral RNA synthesis. This protein is related to the structural pathway of viral particles where it ensures the stability and packaging of the viral genome alongside proteins like the S (spike) and E (envelope) proteins.
The COVID-19 N-Protein is closely associated with the pathogenesis of COVID-19. It triggers an immune response making it a target for antibody detection in serological assays such as the COVID-19 ELISA kit. The N-Protein's interaction with host cell components may influence disease severity by affecting interferon pathways linked to other structural proteins like the S-protein. Understanding these interactions helps in developing therapeutic strategies against COVID-19.
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