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AB276186

COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit

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COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit is a ELISA for the measurement of COVID-19 S-Protein (S1RBD) Human IgM in Human in Biofluids samples.

View Alternative Names

Immunoglobulin mu heavy chain, Immunoglobulin mu heavy chain OU

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ELISA - COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit (AB276186)
  • ELISA

Supplier Data

ELISA - COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit (AB276186)

COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit (ab276186) Calibration curve.

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Human

Results type

Quantitative

Range

1.37 - 1000 U/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit (ab276186) is an in vitro indirect ELISA for the quantitative measurement of human IgM antibody against SARSCoV-2 S1 RBD protein in human serum or dried blood.

Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample and Positive Control, which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of COVID19 S1 RBD protein human IgM antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The Positive Control is from an inactivated serum sample or a dried blood sample which contains SARS-COV-2 S1 RBD protein human IgM antibody. We do not know the exact amount of SARS-COV-2 S1 RBD protein human IgM antibody in the Positive Control sample. The Positive Control can be used as a calibration curve for interpretation purposes in different assays.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab276186-1x96Tests", "assetComponentDetails": [ { "size":"2 x 8 mL", "name":"Stop Solution", "number":"AB276186-CMP07" }, { "size":"1 x 25 mL", "name":"5X Sample Diluent", "number":"AB276186-CMP09" }, { "size":"1 x 1 Unit", "name":"Albumin protein coated 96 well-Microplate", "number":"AB276186-CMP02" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB276186-CMP06" }, { "size":"2 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB276186-CMP08" }, { "size":"1 x 1 Vial", "name":"HRP-Streptavidin Concentrate", "number":"AB276186-CMP05" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB276186-CMP04" }, { "size":"1 x 1 Unit", "name":"SARS-CoV-2 S1 RBD protein coated Microplate", "number":"AB276186-CMP03" }, { "size":"2 x 1 Vial", "name":"Positive Control", "number":"AB276186-CMP01" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human IgM antibody", "number":"AB276186-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab276186-1x96Tests", "assetComponentDetails": [ { "size":"2 x 25 mL", "name":"20X Wash Buffer", "number":"AB276186-CMP04", "productcode":"" }, { "size":"1 x 16 mL", "name":"Stop Solution", "number":"AB276186-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin protein coated 96 well-Microplate", "number":"AB276186-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SARS-CoV-2 S1 RBD protein coated Microplate", "number":"AB276186-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human IgM antibody", "number":"AB276186-CMP10", "productcode":"" }, { "size":"1 x 24 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB276186-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HRP-Streptavidin Concentrate", "number":"AB276186-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Positive Control", "number":"AB276186-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"5X Sample Diluent", "number":"AB276186-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB276186-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The COVID-19 Spike Protein often referred to as SARS-CoV-2 Spike Protein is an important structural protein of the virus with a molecular mass around 180 kDa. This protein facilitates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor which is primarily expressed in the respiratory tract. It's a trimeric protein composed of S1 and S2 subunits. The S1 subunit contains the receptor binding domain while the S2 subunit mediates membrane fusion.
Biological function summary

The spike protein plays an important role in viral infectivity and host range determination. The spike protein is part of the larger viral particle complex that includes other structural proteins like the nucleocapsid protein. Its interaction with host cell receptors triggers conformational changes allowing viral entry and subsequent replication within the host cells. This process is pivotal for the virus's lifecycle.

Pathways

The spike protein is integral to the viral entry pathway a critical step in the viral lifecycle. It intersects with the renin-angiotensin system (RAS) pathway through its interaction with the ACE2 receptor. This interaction not only facilitates viral entry but can also dysregulate the RAS pathway. Additionally the spike protein's activity is related to other viral proteins that assist in replication and assembly such as the envelope protein.

The spike protein is directly linked to COVID-19 the disease caused by SARS-CoV-2 infection. Its interaction with the ACE2 receptor has been associated with severe respiratory illnesses and potentially other conditions like cardiovascular disorders due to its effect on the RAS pathway. The nucleocapsid protein is also important in the virus's pathogenesis working together with the spike protein to establish infection and disease progression.
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Product protocols

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Target data

Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B cell receptor, the secreted form is secreted in large amounts (, PubMed : 16895553).
See full target information IgM
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Product promise

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