Cow IgA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Cow IgA in Cow in Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 12.5 - 400 ng/mL
- Sensitivity
- = 7.009 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
Cow IgA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Cow IgA in Cow in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow
- Range
- 12.5 - 400 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 7.009 ng/mL
- General recovery
- >= 85%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % >= 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's IgA Cow ELISA Kit (ab190516) is a two-site enzyme linked immunoassay (ELISA) for the measurement of IgA in Cow biological samples.
In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.
- Biological function summary
IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.
- Pathways
IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.
- Associated diseases and disorders
Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.
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1 product image
Sandwich ELISA - Cow IgA ELISA Kit (ab190516)
Representative standard curve using ab190516.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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