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AB178638

Coxiella burnetii Phase 1 IgG ELISA Kit (Q-Fever)

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Coxiella burnetii Phase 1 IgG ELISA Kit (Q-Fever) is an indirect ELISA for the qualitative detection of IgG class antibodies against Coxiella burnetii Phase 1 (Q-Fever) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

> 90 %

Assay Platform

Microplate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Abcam's anti-Coxiella burnetii (Q-Fever) Phase 1 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Coxiella burnetii in Human serum.

A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 15, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 15, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178638-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Coxiella burnetii IgG Positive Control", "number":"AB178638-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Coxiella burnetii IgG Negative Control", "number":"AB178638-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178638-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Coxiella burnetii Phase 1 (IgG) Coated Microplate (12 x 8 wells)", "number":"AB178638-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178638-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Coxiella burnetii Anti-IgG HRP Conjugate", "number":"AB178638-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB178638-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178638-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178638-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"Coxiella burnetii IgG Cut-off Control", "number":"AB178638-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB178638-CMP07", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

websiteProtocolBooklet
en

Product promise

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