Cyclic GMP Complete ELISA Kit is a competitive ELISA designed to quantify cyclic GMP.
- Colorimetric competitive ELISA - 405 nm readout - works on any plate reader
- Suitable for cells and tissue treated with 0.1N HCl and culture supernatants, saliva, and serum.
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- Gels (Basel, Switzerland) 9:2023Proniosomal Gel-Loaded Phosphodiesterase Inhibitors (Sildenafil, Vardenafil, and Tadalafil): Prospects for Topical Penile Therapy of Tadalafil for Treatment of Erectile Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoad A Mohamed,Remon Roshdy Rofaeil,Hesham Salem,Mahmoud Elrehany,Yahya I Asiri,Adel Al Fatease,Hamdy AbdelkaderPubMed 37623052
- American journal of physiology. Lung cellular and molecular physiology 324:L677-L6932023Atrophy signaling pathways in respiratory and limb muscles of guinea pigs exposed to chronic cigarette smoke: role of soluble guanylate cyclase stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVíctor Ivo Peinado,Maria Guitart,Isabel Blanco,Olga Tura-Ceide,Tanja Paul,Joan Albert Barberà,Esther BarreiroPubMed 36881560
- PloS one 17:e02794372022Elevated retinal cGMP is not associated with elevated circulating cGMP levels in a canine model of retinitis pigmentosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurence M Occelli,Kelian Sun,Paige A Winkler,Brandy J Morgan,Simon M Petersen-JonesPubMed 36584140
- Nature communications 13:664720222'-5' oligoadenylate synthetase‑like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Kyeong Kim,Sejin Jeon,Seonjun Park,Seong-Keun Sonn,Seungwoon Seo,Joowon Suh,Jing Jin,Hyae Yon Kweon,Sinai Kim,Shin Hye Moon,Okhee Kweon,Bon-Hyeock Koo,Nayoung Kim,Hae-Ock Lee,Young-Myeong Kim,Young-Joon Kim,Sung Ho Park,Goo Taeg OhPubMed 36333342
- Frontiers in dental medicine 9:2022Control of Orthodontic Tooth Movement by Nitric Oxide Releasing Nanoparticles in Sprague-Dawley Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerrick Crawford,Tommy C Lau,Megan C Frost,Nan E HatchPubMed 36081866
- Frontiers in cell and developmental biology 9:7741852022Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase Activation of PDE5A-cGMP-PKG in Cattle Heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingjuan Gu,Xinyu Zhou,Lin Zhu,Yajie Gao,Li Gao,Chunling Bai,Lei Yang,Guangpeng LiPubMed 35155444
- BMJ open diabetes research & care 8:2020Rebalancing glucolipid metabolism and gut microbiome dysbiosis by nitrate-dependent alleviation of high-fat diet-induced obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinsha Ma,Liang Hu,Luyuan Jin,Jiangyi Wang,Xiangchun Li,Weili Wang,Shimin Chang,Chunmei Zhang,Jingsong Wang,Songlin WangPubMed 32843498
- World journal of gastroenterology 24:4356-43682018Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenise Schaffner,Adhara Lazaro,Peter Deibert,Peter Hasselblatt,Patrick Stoll,Lisa Fauth,Manfred W Baumstark,Irmgard Merfort,Annette Schmitt-Graeff,Wolfgang KreiselPubMed 30344420
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Competitive
- Assay time
- 3h
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Alternative names
Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate
What's included?
Recommended products
Cyclic GMP Complete ELISA Kit is a competitive ELISA designed to quantify cyclic GMP.
- Colorimetric competitive ELISA - 405 nm readout - works on any plate reader
- Suitable for cells and tissue treated with 0.1N HCl and culture supernatants, saliva, and serum.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Competitive
- Assay time
- 3h
- Assay Platform
- Microplate
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101.3 | Range |
Sample type Urine | Average % = 97.7 | Range |
Sample type Saliva | Average % = 102.9 | Range |
Sample type EDTA Plasma | Average % = 115 | Range |
Sample type Heparin Plasma | Average % = 104.4 | Range |
Sample type Tissue Culture Media | Average % = 101.7 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Cyclic GMP Complete in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP Complete in cells and tissue treated with 0.1N HCl, in addition to culture supernatants, saliva, and serum.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.
Guanosine 3',5'-cyclic monophosphate (cyclic GMP; cGMP) was identified in 1963. It has been shown to be present at levels typically.10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme.guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin, and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators niroprusside, nitroglycerin, sodium nitrate, and nitric oxide.(NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP) that relax smooth muscle also increase cGMP concentrations. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.
Cross Reactivities:
| Compound | Cross Reactivity |
| cGMP | 100% |
| GMP | <0.001% |
| GTP | <0.001% |
| cAMP | <0.001% |
| AMP | <0.001% |
| ATP | <0.001% |
| cUMP | <0.001% |
| CTP | <0.001% |
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cyclic guanosine monophosphate commonly known as cGMP is a second messenger important in various physiological processes. It has a molecular mass of 345.2 g/mol. cGMP is produced as a result of the conversion of GTP by the enzyme guanylate cyclase and is degraded by phosphodiesterases. cGMP is expressed in several tissues including the brain heart and vascular smooth muscle cells. It plays significant roles in cell signaling and cellular communication.
- Biological function summary
Cyclic guanosine monophosphate influences vasodilation and neurotransmission. It functions within cellular signaling complexes and often partners with proteins such as protein kinases to achieve its biological effects. cGMP facilitates the relaxation of smooth muscle tissues impacting cardiovascular health by regulating blood vessel dilation. Similarly it modulates neural signaling affecting processes like memory formation and vision.
- Pathways
CGMP plays an important role in the nitric oxide signaling pathway and the atrial natriuretic peptide (ANP) pathway. Within the nitric oxide signaling pathway cGMP works in coordination with nitric oxide synthase and protein kinase G to mediate vasodilation. In the ANP pathway cGMP acts downstream of guanylate cyclase-linked receptors in maintaining cardiovascular and renal functions interconnecting with proteins like atrial natriuretic peptide to regulate blood pressure and volume.
- Associated diseases and disorders
Cyclic guanosine monophosphate is associated with hypertension and erectile dysfunction. Its role in vascular regulation links it to hypertension where decreased cGMP levels can elevate blood pressure. Additionally in erectile dysfunction cGMP imbalance disrupts proper blood flow regulation necessary for erection. Phosphodiesterase inhibitors such as sildenafil target cGMP pathways to restore normal function in these conditions highlighting the protein’s involvement in disease treatments.
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2 product images
Competitive ELISA - Cyclic GMP Complete ELISA Kit (ab133052)
Typical Standard Curve - Non-Acetylated Format.
Representative Standard Curve using ab133052. Non-Acetylated Format.
Competitive ELISA - Cyclic GMP Complete ELISA Kit (ab133052)
Typical Standard Curve - Acetylated Format.
Representative Standard Curve using ab133052. Acetylated Format.
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