Cyclic GMP Direct ELISA Kit is a Competitive ELISA for the measurement of Cyclic GMP Direct in in Cell/Tissue Extracts samples.
View Alternative Names
Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate, cGMP
- cELISA
Supplier Data
Competitive ELISA - Cyclic GMP Direct ELISA Kit (AB133027)
Representative Standard Curve using ab133027.
- cELISA
Supplier Data
Competitive ELISA - Cyclic GMP Direct ELISA Kit (AB133027)
Representative Standard Curve using ab133027
Reactivity data
Product details
Abcam's Cyclic GMP (cGMP) Direct in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP in tissue culture media treated with 0.1 M HCl.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.
Guanosine 3', 5'-cyclic monophosphate (cyclic GMP; cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also increase cGMP levels. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells. Bioactive peptides like atrial natriuretic peptide (ANP) also increase cGMP concentrations.
Cross Reactivity
| Compound | % Cross Reactivity |
| cGMP | 100 |
| GMP | <0.001 |
| GTP | <0.001 |
| cAMP | <0.001 |
| AMP | <0.001 |
| ATP | <0.001 |
| cUMP | <0.001 |
| CTP | <0.001 |
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cyclic guanosine monophosphate influences vasodilation and neurotransmission. It functions within cellular signaling complexes and often partners with proteins such as protein kinases to achieve its biological effects. cGMP facilitates the relaxation of smooth muscle tissues impacting cardiovascular health by regulating blood vessel dilation. Similarly it modulates neural signaling affecting processes like memory formation and vision.
Pathways
CGMP plays an important role in the nitric oxide signaling pathway and the atrial natriuretic peptide (ANP) pathway. Within the nitric oxide signaling pathway cGMP works in coordination with nitric oxide synthase and protein kinase G to mediate vasodilation. In the ANP pathway cGMP acts downstream of guanylate cyclase-linked receptors in maintaining cardiovascular and renal functions interconnecting with proteins like atrial natriuretic peptide to regulate blood pressure and volume.
Product protocols
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Publications (1)
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International journal of molecular sciences 20: PubMed31248114
2019
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