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AB108669

DHEA sulfate (DHEA-S) ELISA Kit

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(6 Publications)

DHEA sulfate (DHEA-S) ELISA Kit is a competitive ELISA designed to quantify DHEA sulfate (DHEA-S) with a sensitivity of 0.04 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.1 - 10 µg/ml
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Competitive ELISA - DHEA sulfate (DHEA-S) ELISA Kit (AB108669)
  • cELISA

Unknown

Competitive ELISA - DHEA sulfate (DHEA-S) ELISA Kit (AB108669)

Representative Standard Curve using ab108669

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.04 ng/mL

Range

0.1 - 10 µg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.

A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Control", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10.4" }, { "reproducibilityType": "Intra", "sample": "Control", "replicates": 64, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 7.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108669-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108669-CMP15", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foils", "number":"AB108669-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 0 – 0 µg/mL", "number":"AB108669-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108669-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 4 – 4.0 µg/mL", "number":"AB108669-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells)", "number":"AB108669-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Wash Solution", "number":"AB108669-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108669-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control B", "number":"AB108669-CMP16", "productcode":"" }, { "size":"1 x 100 mL", "name":"Serum Diluent", "number":"AB108669-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 3 – 1.0 µg/mL", "number":"AB108669-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 2 – 0.4 µg/mL", "number":"AB108669-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Control A", "number":"AB108669-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 5 – 10.0 µg/mL", "number":"AB108669-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"DHEA sulfate Standard 1 – 0.1 µg/mL", "number":"AB108669-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"DHEA sulfate-HRP Conjugate", "number":"AB108669-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dehydroepiandrosterone sulfate (DHEA-S) also known as DHEA sulphate is a sulfated steroid hormone with a mass of approximately 368.5 Da. DHEA-S is predominantly expressed in the adrenal glands though small amounts can be found in the gonads and brain. It is derived from dehydroepiandrosterone (DHEA) through the action of sulfotransferase enzymes. DHEA-S can be measured through various methods including DHEA sulfate ELISA to assess its concentration in biological samples providing insights into adrenal function and related conditions.
Biological function summary

DHEA-S plays an important role in the production of androgens and estrogens which are significant sex hormones. It acts as a reservoir that can be transformed into these active hormones as needed and this conversion occurs in peripheral tissues. DHEA-S does not operate as part of a stable complex but relies on enzymatic conversion in pathways to exert its effects. Its levels naturally decline with age suggesting its involvement in aging processes and age-related functions making it an interesting target for research on health and longevity.

Pathways

DHEA-S is involved in the steroidogenesis pathway where it interconnects with other steroid hormones like testosterone and estradiol. This pathway is essential for the synthesis and regulation of various steroid hormones. DHEA-S is also connected with the adrenal steroid synthesis pathway and it shares metabolic relationships with enzymes such as 3β-hydroxysteroid dehydrogenase and aromatase which aid in the interconversion of steroid precursors to active hormones.

DHEA-S links to several conditions including adrenal insufficiency and polycystic ovary syndrome (PCOS). In adrenal insufficiency it serves as a marker for reduced adrenal function where lower levels indicate impaired hormone synthesis. In PCOS alterations in DHEA-S levels are common as it can lead to excessive androgen production contributing to symptom development. DHEA-S and its levels can also interact with insulin resistance pathways where the interplay with proteins involved in glucose metabolism becomes significant.
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Product protocols

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Target data

See full target information Dehydroepiandrosterone sulfate
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Toxicology reports 14:102009 PubMed40226810

2025

Potential amelioration of liver function by low-dose tolvaptan in heart failure patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Mino,Kohei Hoshikawa,Takafumi Naito,Shunta Akutsu,Yumi Imoto,Emi Nakatsugawa,Masao Saotome,Yuichiro Maekawa,Junichi Kawakami

Frontiers in immunology 14:1137089 PubMed37275915

2023

ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 gut microbiota and microbial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Man Luo,Yuanyuan Chen,Xiangyang Pan,Hongmei Chen,Lang Fan,Yi Wen

Molecular medicine reports 26: PubMed35656892

2022

CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxiao Wen,Xiaoqing Dou,Shuzhen Zhang,Bin Wang,Jiangyan Xu,Wenchao Zhang,Feifei Wang

Frontiers in aging neuroscience 12:155 PubMed32581767

2020

Prevention of Early Alzheimer's Disease by Erinacine A-Enriched Mycelia Pilot Double-Blind Placebo-Controlled Study.

Applications

Unspecified application

Species

Unspecified reactive species

I-Chen Li,Han-Hsin Chang,Chuan-Han Lin,Wan-Ping Chen,Tsung-Han Lu,Li-Ya Lee,Yu-Wen Chen,Yen-Po Chen,Chin-Chu Chen,David Pei-Cheng Lin

Biogerontology 20:823-835 PubMed31396798

2019

High perceived stress in women is linked to oxidation, inflammation and immunosenescence.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Martínez de Toda,Lara Miguélez,León Siboni,Carmen Vida,Mónica De la Fuente

Pain medicine (Malden, Mass.) 18:846-855 PubMed27498549

2016

Different DHEA-S Levels and Response Patterns in Individuals with Chronic Neck Pain, Compared with a Pain Free Group-a Pilot Study.

Applications

cELISA

Species

Human

A Grimby-Ekman,B Ghafouri,H Sandén,B Larsson,B Gerdle
View all publications
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