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AB274408

Dog Thymidine Kinase ELISA Kit (TK1)

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Dog Thymidine Kinase ELISA Kit (TK1) is a Sandwich (quantitative) ELISA for the measurement of Dog Thymidine Kinase (TK1) in Dog in Biofluids samples.
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Sandwich ELISA - Dog Thymidine Kinase ELISA Kit (TK1) (AB274408)
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Supplier Data

Sandwich ELISA - Dog Thymidine Kinase ELISA Kit (TK1) (AB274408)

Example standard curve using Canine Thymidine Kinase (TK1) ELISA Kit (ab274408).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Dog

Assay type

Sandwich (quantitative)

Range

0.16 - 5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

The Dog Thymidine Kinase ELISA Kit (TK1) (ab274408) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring TK1 in dog biological samples.
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 1 (TK1) also known as Thymidine Kinase isoform A is an enzyme involved in the phosphorylation of thymidine into thymidine monophosphate an important component in DNA synthesis. TK1 has a molecular weight of approximately 25 kDa. It is predominantly expressed in proliferating cells with expression levels peaking during the S phase of the cell cycle. You often find TK1 in tissues with high cell division rates like bone marrow and lymphatic tissues.
Biological function summary

This enzyme is critical for DNA replication and repair by providing deoxythymidine triphosphate (dTTP) a deoxynucleoside triphosphate. TK1 operates as a monomer but can form a tetrameric complex in its active state which enhances its affinity for substrates. TK1 can process various substrates including thymidine and thymidine analogs such as 5-bromo-2'-deoxyuridine (BrdU) and EdU. These substrates are vital in molecular biology as they help in measuring DNA synthesis in cell proliferation studies.

Pathways

The function of TK1 is central to the salvage pathway of nucleotide synthesis. This pathway allows recycling of thymidine from degraded DNA which is particularly important for rapidly dividing cells that need to ensure a sufficient supply of nucleotides. TK1 acts alongside other enzymes such as thymidylate synthase and ribonucleotide reductase to maintain nucleotide balance within the cell ensuring proper DNA synthesis and cellular proliferation.

TK1 levels often increase in cancerous tissues due to their elevated cell proliferation rates. Serum TK1 is a biomarker for various cancers like leukemia and breast cancer. Studies show a correlation between high TK1 activity and tumor aggressiveness and progression. Additionally interactions of TK1 with the protein p53 a tumor suppressor show that defects in TK1 regulation can contribute to genomic instability highlighting its role in oncogenesis.
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Product protocols

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Target data

See full target information TK1
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websiteProtocolBooklet
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Complementary Products

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Secondary Antibodies

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Biochemicals

AB142567

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Proteins & Peptides

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