Dopamine ELISA Kit

Product details

Dopamine ELISA kit ab285238 is a competitive ELISA designed to quantify dopamine (DA) with a sensitivity of <0.938 ng/mL.

How the assay works

Dopamine ELISA kit ab285238 is a competitive ELISA. There are 2 types of competitive ELISAs:

• antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
• antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Dopamine ELISA kit ab285238 protocol summary

1. Add standards or samples to the wells, incubate 2 hrs
2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately