AB269543

DSCAM ELISA Kit

Recombinant
SimpleStep
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DSCAM ELISA Kit is a single-wash 90-min Simplestep used to quantify DSCAM with a sensitivity of 38.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cell adhesion molecule DSCAM, CHD2, Down syndrome cell adhesion molecule, DSCAM

2 Images

Sandwich ELISA - DSCAM ELISA Kit (AB269543)

sELISA

Supplier Data

Sandwich ELISA - DSCAM ELISA Kit (AB269543)

Interpolated concentrations of native DSCAM in mouse and rat brain homogenate extract based on a 1,000 μg/mL extract load.

The concentrations of DSCAM were measured in duplicate and interpolated from the DSCAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DSCAM concentration was determined to be 1,792.70 pg/ml in 1,000 μg/mL mouse brain homogenate extract and 1,651.44 pg/ml in 1,000 μg/mL rat brain homogenate extract.

sELISA

Supplier Data

Sandwich ELISA - DSCAM ELISA Kit (AB269543)

Example of DSCAM standard curve in 1X Cell Extraction Buffer PTR.

The DSCAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse, Rat, Human

Assay type

Sandwich (quantitative)

Sensitivity

= 38.39 pg/mL

Range

203.13 - 13000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

DSCAM ELISA Kit (ab269543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DSCAM protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate DSCAM with 38.39 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

DSCAM is an adhesion protein expressed in the highest level in the fetal brain, although DSCAM is also expressed in other tissues and in adults. DSCAM is involved in neurogenesis and self-avoidance, but also in immunity in invertebrates. Overexpression of DSCAM in the fetal brain leads to Down syndrome, and altered DSCAM levels have been observed in PTSD, bipolar disorder, depression and Alzheimer disease. DSCAM has high amino acid sequence identity between human, mouse and rat. The standard protein in this product is human DSCAM.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": null, "average": "= 101" }, { "sample": "Cell Lysate", "range": null, "average": "= 99" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269543-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269543-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269543-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB269543-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269543-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X DSCAM Capture Antibody", "number":"AB269543-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"DSCAM Lyophilized Recombinant Protein", "number":"AB269543-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269543-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X DSCAM Detector Antibody", "number":"AB269543-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269543-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB269543-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB269543-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269543-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dscam known as Down Syndrome Cell Adhesion Molecule is a protein with a mass of around 230 kDa. It functions as a cell adhesion molecule and is involved in axon guidance. Dscam is expressed in various tissues with high levels in the nervous system particularly during developmental stages. The protein plays a role in neural development by ensuring proper neuronal connections.

Biological function summary

Dscam contributes to neuronal differentiation and synaptic targeting. It belongs to the immunoglobulin superfamily and is involved in forming non-covalent complexes with other cell adhesion molecules. The protein facilitates homophilic binding which regulates axonal and dendritic branching. This process ensures neural circuits form correctly impacting brain development and function.

Pathways

Dscam participates in the neural development pathway which includes interactions with the Robo receptor affecting axonal migration and guidance. The protein also links to the slit-Robo pathway playing a substantial role in neural patterning. These interactions ensure the precise wiring required for functional neural networks influencing how neural signals propagate.

Dscam's dysregulation connects to neurological conditions like Down syndrome and autism spectrum disorders. It interacts with proteins involved in these disorders like the ROBO proteins which mediate axon guidance. Accumulating evidence suggests that altered Dscam expression impairs neuronal connectivity contributing to the pathophysiology of these conditions.

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Product protocols

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Target data

Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed : 10925149). Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC (By similarity). In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding (PubMed : 18585357, PubMed : 19196994). Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38 (PubMed : 18585357, PubMed : 19196994). Adhesion molecule that promotes lamina-specific synaptic connections in the retina : expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).

See full target information Down syndrome cell adhesion molecule

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DSCAM ELISA Kit

Sandwich ELISA - DSCAM ELISA Kit (AB269543)

Sandwich ELISA - DSCAM ELISA Kit (AB269543)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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