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EIF2S1 ELISA Kit (pS51) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of EIF2S1 (pS51) in Cell Lysate samples.
Colorimetric
Cell Lysate
Sandwich (quantitative)
Human
51.56 - 3300 pg/mL
1h 30m
= 9 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
EIF2S1 ELISA Kit (pS51) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of EIF2S1 (pS51) in Cell Lysate samples.
Colorimetric
Cell Lysate
Sandwich (quantitative)
Human
51.56 - 3300 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 9 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.8 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 4.7 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell Lysate | Average % = 106 | Range 99 - 114 % |
Blue Ice
+4°C
+4°C
EIF2S1 ELISA kit (ab282296) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EIF2S1 protein in human cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate EIF2S1 with 9 units/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, plays a key role in the initiation of protein synthesis. Dysregulation of EIF2S1 plays a part in the development of type II diabetes and ischemic injury. The immunogen from which the antibodies in this kit were raised represents the full-length mature protein. This protein shares homology across Mammalia, and therefore is expected to cross-react with many species. The protein standard in this product is recombinant human EIF2S1 ligated with peptide containing phosphorylated serine 51.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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