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AB282296

EIF2S1 ELISA Kit (pS51)

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EIF2S1 ELISA Kit (pS51) is a single-wash 90-min Simplestep used to quantify EIF2S1 (pS51) with a sensitivity of 9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)
  • ELISA

Supplier Data

ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)
Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)
  • sELISA

Supplier Data

Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)

The EIF2S1(pS51) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)
  • sELISA

Supplier Data

Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)

Cells were treated with 50 nM pervanadate for 30 minutes, scraped and buffer exchanged into PBS + 20 mM NaF, followed by protein extraction. Mock control cells accompanied cell treatment. Concentrations of EIF2S1(pS51) were measured in duplicate and interpolated against the standard curve. Results show the mean EIF2S1(pS51) concentration was 68.55 pg/mL in 7.5 ug/mL HeLa control cells, 876.09 pg/mL in 7.5 ug/mL HeLa + Calyculin, 173.27 pg/mL in 12.5 ug/mL NIH-3T3 control, 919.27 pg/mL in 12.5 ug/mL NIH-3T3 + Calyculin, 112.07 pg/mL in 12.5 ug/mL C6 control, and 813.39 pg/mL in 7.5 ug/mL C6 + Calyculin.

Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)
  • sELISA

Supplier Data

Sandwich ELISA - EIF2S1 ELISA Kit (pS51) (AB282296)

The concentrations of EIF2S1(pS51) were measured in duplicate and interpolated from the EIF2S1(pS51) standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EIF2S1(pS51) concentration was determined to be 3,070.84 pg/mL in HeLa + Calyculin cell extract, 2,802.47 pg/mL in NIH-3T3 + Calyculin cell extract and 2,650.15 pg/mL in C6 + Calyculin cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9 pg/mL

Range

51.56 - 3300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

EIF2S1 ELISA Kit (pS51) ab282296 is a rapid single-wash 90-min Sandwich ELISA to measure EIF2S1 (pS51)in cell lysate. This SimpleStep sensitivity is 9 pg/mL.

How the assay works

EIF2S1 (pS51)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

EIF2S1 ELISA Kit (pS51) ab282296 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, plays a key role in the initiation of protein synthesis. Dysregulation of EIF2S1 plays a part in the development of type II diabetes and ischemic injury. The immunogen from which the antibodies in this kit were raised represents the full-length mature protein. This protein shares homology across Mammalia, and therefore is expected to cross-react with many species. The protein standard in this product is recombinant human EIF2S1 ligated with peptide containing phosphorylated serine 51.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "99 - 114 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S1 also called eIF2? is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary

EIF2? acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2? leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2? forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.

Pathways

EIF2? is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2? is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2? plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.

EIF2? connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2? are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2? signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2? in these diseases highlighting eIF2?'s involvement in critical pathological processes.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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