AB133030

Endothelin 1 ELISA Kit

Name: Endothelin 1 ELISA Kit (ab133030) | Abcam
Brand: Abcam Limited
SKU: AB133030
Price: 980 USD
Availability: InStock

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(38 Publications)

Endothelin 1 ELISA Kit is a sandwich ELISA designed to quantify Endothelin 1 with a sensitivity of 0.41 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.78 - 100 pg/mL
- Cited in over 20 publications

View Alternative Names

Endothelin-1, Preproendothelin-1, PPET1, EDN1

2 Images

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

sELISA

Supplier Data

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Representative Standard Curve using ab133030.

sELISA

Lab

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Sample results.

Endothelin 1 measured in biological fluids of healthy species showing quantity (pg) per mL of tested sample. Results obtained from samples that were not extracted. Samples diluted 1-9 fold.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Rat, Rabbit, Cow, Dog, Human, Pig

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 pg/mL

Range

0.78 - 100 pg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Endothelin 1 ELISA Kit ab133030 is a sandwich ELISA to measure Endothelin 1 in serum, plasma, cell culture supernatant with a sensitivity of 0.41 pg/ml.

How the assay works

Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Endothelin 1 ELISA Kit ab133030 protocol summary

1. Add standard or sample to each well used. Incubate wells.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate wells.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well. Immediately begin recording the color development.

Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

Cross Reactivity

Compound	Cross Reactivity
Endothelin 1 (ET-1)	100
ET-2	21
ET-3	3.6
Human Big ET-1	<0.1
Rat Big ET-1	<0.1
Human Big ET-2	<0.1
Human Big ET-3 Amide	<0.1

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "1.2 pg/mL", "standardDeviation": null, "coefficientOfVariability": "15.3" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "2.5 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Buffer", "replicates": 20, "mean": "35.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "35.9 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "1.1 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Buffer", "replicates": 20, "mean": "2.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.9" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.

Biological function summary

ET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.

Pathways

ET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.

ET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.

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Product protocols

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Target data

Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed : 19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437). Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments (By similarity). NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity).

See full target information EDN1

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 15:101251 PubMed40521370

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Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome.

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Yuan Tian,Dan Zhang,Huaian Yang,Xiaoli Zhang,Shengqun Xu

Brain communications 7:fcaf070 PubMed40008326

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Structural brain changes in post-COVID condition and its relationship with cognitive impairment.

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Laura Pacheco-Jaime,Carla Garcia-Vicente,Mar Ariza,Neus Cano,Maite Garolera,Lourdes Carreras-Vidal,Ignacio Roura,Clara Capdevila-Lacasa,Javier Oltra,Jèssica Pardo,Cristina Martín-Barceló,Anna Campabadal,Roser Sala-Llonch,Núria Bargalló,Cristian Barrué,Javier Bejar,Claudio U Cortés,Carme Junqué,Bàrbara Segura

Toxicology reports 13:101851 PubMed39717857

2024

Comparative in vitro toxicity of compositionally distinct thermal spray particulates in human bronchial cells.

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E S Burns,R E Harner,V Kodali,A A Afshari,J M Antonini,S S Leonard

Biomolecules & biomedicine 25:682-692 PubMed39319864

2024

Regulatory role and molecular mechanism of METTL14 in vascular endothelial cell injury in preeclampsia.

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Huafang Wei,Lin Liang,Chengwen Song,Ming Tong,Xiang Xu

Clinical science (London, England : 1979) 138:1131-1150 PubMed39282930

2024

Identifying mitigating strategies for endothelial cell dysfunction and hypertension in response to VEGF receptor inhibitors.

Applications

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Species

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Nicholas D Camarda,Qing Lu,Dawn M Meola,Joshua J Man,Zeyuan Song,Richard J Travers,Katherine E Lopez,Sarah N Powers,Malvina Papanastasiou,Katherine C DeRuff,James Mullahoo,Shawn B Egri,Desiree Davison,Paola Sebastiani,Scott T Eblen,Rachel Buchsbaum,Gordon S Huggins,Cheryl A London,Jacob D Jaffe,Jenica N Upshaw,Vicky K Yang,Iris Z Jaffe

Nature microbiology 9:2538-2552 PubMed39261580

2024

Blockade of endothelin receptors mitigates SARS-CoV-2-induced osteoarthritis.

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Species

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Man Ting Au,Junguo Ni,Kaiming Tang,Wei Wang,Lanlan Zhang,Hantang Wang,Fangyi Zhao,Zhan Li,Peng Luo,Lawrence Chun-Man Lau,Ping-Keung Chan,Cuiting Luo,Bo Zhou,Lin Zhu,Charlie Yuli Zhang,Tianshu Jiang,Marianne Lauwers,Jasper Fuk-Woo Chan,Shuofeng Yuan,Chunyi Wen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23092 PubMed37482902

2023

Blockade of the mineralocorticoid receptor improves markers of human endothelial cell dysfunction and hematological indices in a mouse model of sickle cell disease.

Applications

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Species

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Alicia Rivera,Christopher Vega,Arelys Ramos-Rivera,Enrique R Maldonado,Gregory N Prado,Hope E Karnes,Yuri A Fesko,L Michael Snyder,Seth L Alper,Jose R Romero

International journal of molecular sciences 24: PubMed37108136

2023

Dual Blockade of TGF-β Receptor and Endothelin Receptor Synergistically Inhibits Angiotensin II-Induced Myofibroblast Differentiation: Role of ATR/G-Mediated TGF-β1 and ET-1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ratchanee Duangrat,Warisara Parichatikanond,Supachoke Mangmool

BMC endocrine disorders 22:293 PubMed36434695

2022

Novel sedentary cage induced sedentariness in rats: evidence from relevant biomarkers.

Applications

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Species

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Quadri K Alabi,Rufus O Akomolafe

Diabetes & vascular disease research 19:14791641221131788 PubMed36357361

2022

Monitoring and recovery of hyperglycaemia-induced endothelial dysfunction with rheopheresis in diabetic lower extremity ulceration with hyperviscosity.

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Unspecified application

Species

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Kristóf Gál,Georgina Asbóth,Melinda Vass,Attila Bíró,Arnold Markovich,Judit Homoki,Gábor Fidler,Melinda Paholcsek,Zoltán Cziáky,Norbert Németh,Judit Remenyik,Pál Soltész

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Endothelin 1 ELISA Kit

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Sandwich ELISA - Endothelin 1 ELISA Kit (AB133030)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise