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AB155466

Equine IL-10 ELISA Kit

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(2 Publications)

Equine IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Equine IL-10 in Horse in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
Sandwich ELISA - Equine IL-10 ELISA Kit (AB155466)
  • sELISA

Unknown

Sandwich ELISA - Equine IL-10 ELISA Kit (AB155466)

Representative standard curve using ab155466 - Assay Diluent B.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Horse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 90 pg/mL

Range

0.1 - 25 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Equine ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of equine IL-10 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Equine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-equine IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 112 %", "average": "= 104.9" }, { "sample": "Plasma", "range": "86 - 102 %", "average": "= 94.01" }, { "sample": "Cell culture media", "range": "90 - 105 %", "average": "= 97.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155466-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155466-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155466-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 strips x 8 wells )", "number":"AB155466-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155466-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155466-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-10 Standard equine(recombinant) (lyophilized)", "number":"AB155466-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155466-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody IL-10 : biotinylated anti-equine IL-10", "number":"AB155466-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155466-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 32:101360 PubMed39703903

2024

Ocular toxicity, distribution, and shedding of intravitreal AAV-eqIL-10 in horses.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Young,Tomoko Hasegawa,Naveen Vridhachalam,Nichol Henderson,Jacklyn H Salmon,Trace F McCall,Matthew L Hirsch,Brian C Gilger

Frontiers in veterinary science 9:843131 PubMed35252428

2022

Case Report: Repeated Intralesional Injections of Autologous Mesenchymal Stem Cells Combined With Platelet-Rich Plasma for Superficial Digital Flexor Tendon Healing in a Show Jumping Horse.

Applications

sELISA

Species

Horse

Luca Melotti,Anna Carolo,Noha Elshazly,Filippo Boesso,Laura Da Dalt,Gianfranco Gabai,Anna Perazzi,Ilaria Iacopetti,Marco Patruno
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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