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AB176641

ERK1/2 ELISA Kit

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(7 Publications)

ERK1/2 ELISA Kit is a single-wash 90-min Simplestep used to quantify ERK1/2 with a sensitivity of 0.6 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

View Alternative Names

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

3 Images
Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)

Example of a typical ERK1/2 Total control lysate dilution series in 1X Cell Extraction Buffer PTR. The ERK1/2 lysate dilution series was prepared as described in Section 10. Raw data values are shown in the table.

Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)

HeLa cells were seeded at 40K cells/well in a 96-well tissue culture plate and incubated overnight. Cells were treated with AG1478 at the indicated concentrations for 2.5 hours and then treated with 1 ng/mL of EGF for 10 minutes prior to cell lysis. Data from triplicate measurements of ERK1/2 (pT202/Y204) are plotted and compared against ERK1/2 Total protein levels.

Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)
  • sELISA

Supplier Data

Sandwich ELISA - ERK1/2 ELISA Kit (AB176641)

Cell line analysis for ERK1/2 Total from preparations of 17,500 cells/well cell extracts. Data from triplicate measurements (mean +/- SD) are plotted.

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate

Reacts with

Mouse, Human

Assay type

Semi-quantitative

Sensitivity

= 0.6 ng/mL

Range

0.6 - 60 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's ERK1/2 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ERK1/2 protein in Human and mouse cells.

The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "A431 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "A431 Extract", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab176641-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB176641-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ERK1/2 (Total) Capture Antibody", "number":"AB176641-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB176641-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB176641-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"ERK1/2 (Total) Detector Antibody", "number":"AB176641-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB176641-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB176641-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized ERK1/2 Control Lysate", "number":"AB176641-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB176641-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB176641-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as MAPK3 and MAPK1 respectively are protein kinases involved in the mitogen-activated protein kinase (MAPK) signaling pathway. They share high sequence identity and exhibit similar functions. ERK1 has a molecular weight of approximately 44 kDa while ERK2 weighs around 42 kDa. Both are expressed ubiquitously in various tissues playing roles in diverse cellular processes. These proteins often detected through ERK1/2 western blot analyses present similar ERK protein size and ERK molecular weight characteristics relevant during protein expression studies.
Biological function summary

ERK1 and ERK2 play key roles in cell proliferation differentiation and survival. They form part of a cascade that includes upstream activators such as MEK1/2 and downstream targets including transcription factors. As components of the MAPK signaling complex ERK1/2 regulate gene expression through phosphorylation events impacting cellular responses to various stimuli. Their activation often hinges on growth factors cytokines and stress signals facilitating cellular adaptation to environmental changes.

Pathways

Regarding pathways ERK1/2 sit within the MAPK/ERK pathway and are significant in the Ras/Raf/MEK/ERK cascade one of the foremost signaling mechanisms in cells. They interact with several proteins including Ras and Raf which modulate their activation. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus impacting gene regulation and cell fate decisions. ERK1/2 proteins therefore serve as critical nodes linking extracellular signals to cellular responses ensuring balanced cell function.

ERK1/2 play significant roles in cancer and neurodegenerative diseases. Their overactivation is often linked to oncogenic processes contributing to cell proliferation in cancers. Mutations or dysregulation within the MAPK/ERK pathway including ERK1/2 frequently associate with tumorigenesis. Additionally in neurodegenerative disorders like Alzheimer's disease altered ERK1/2 activity may impact neuronal survival and function often through interaction with amyloid-beta and tau proteins further illustrating their importance in disease pathophysiology.
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Product protocols

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
See full target information MAPK3

Additional targets

MAPK1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in psychiatry 12:678090 PubMed34093287

2021

GABA Alpha 2,3 Modulation Improves Select Phenotypes in a Mouse Model of Fragile X Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Tori L Schaefer,Amy A Ashworth,Durgesh Tiwari,Madison P Tomasek,Emma V Parkins,Angela R White,Andrew Snider,Matthew H Davenport,Lindsay M Grainger,Robert A Becker,Chandler K Robinson,Rishav Mukherjee,Michael T Williams,Jay R Gibson,Kimberly M Huber,Christina Gross,Craig A Erickson

OncoTargets and therapy 13:4431-4444 PubMed32547074

2020

The Long Noncoding RNA ZFAS1 Potentiates the Development of Hepatocellular Carcinoma via the microRNA-624/MDK/ERK/JNK/P38 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Duan,Caiyan Li,Fan Wang,Fei Han,Ling Zhu

International journal of environmental research an 17: PubMed31906378

2020

Bisphenol a Exposure, DNA Methylation, and Asthma in Children.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Feng Yang,Wilfried J J Karmaus,Chen-Chang Yang,Mei-Lien Chen,I-Jen Wang

International journal of molecular medicine 42:3247-3255 PubMed30320369

2018

Evodiamine alleviates severe pneumonia induced by methicillin-susceptible Staphylococcus aureus following cytomegalovirus reactivation through suppressing NF-κB and MAPKs.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chen,Shujun Zhou,Hui Li

Oxidative medicine and cellular longevity 2017:5290161 PubMed28904737

2017

Anticataractogenesis and Antiretinopathy Effects of the Novel Protective Agent Containing the Combined Extract of Mango and Vietnamese Coriander in STZ-Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jintanaporn Wattanathorn,Paphaphat Thiraphatthanavong,Wipawee Thukham-Mee,Supaporn Muchimapura,Panakaporn Wannanond,Terdthai Tong-Un

Journal of neurodevelopmental disorders 9:6 PubMed28616095

2017

Acamprosate in a mouse model of fragile X syndrome: modulation of spontaneous cortical activity, ERK1/2 activation, locomotor behavior, and anxiety.

Applications

ELISA

Species

Mouse

Tori L Schaefer,Matthew H Davenport,Lindsay M Grainger,Chandler K Robinson,Anthony T Earnheart,Melinda S Stegman,Anna L Lang,Amy A Ashworth,Gemma Molinaro,Kimberly M Huber,Craig A Erickson

Circulation research 119:853-64 PubMed27444755

2016

Proliferation and Recruitment Contribute to Myocardial Macrophage Expansion in Chronic Heart Failure.

Applications

sELISA

Species

Mouse

Hendrik B Sager,Maarten Hulsmans,Kory J Lavine,Marina B Moreira,Timo Heidt,Gabriel Courties,Yuan Sun,Yoshiko Iwamoto,Benoit Tricot,Omar F Khan,James E Dahlman,Anna Borodovsky,Kevin Fitzgerald,Daniel G Anderson,Ralph Weissleder,Peter Libby,Filip K Swirski,Matthias Nahrendorf
View all publications
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