ERK1/2 (pT202/Y204) ELISA Kit is a single-wash 90-min Simplestep used to quantify ERK1/2 (pT202/Y204) with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 10 citations
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- Disease models & mechanisms 16:2023Vemurafenib improves muscle histopathology in a mouse model of LAMA2-related congenital muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAriany Oliveira-Santos,Marisela Dagda,Jennifer Wittmann,Robert Smalley,Dean J BurkinPubMed 37021539
- Experimental and therapeutic medicine 25:2602023ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunjie Sui,Dan ZhouPubMed 37153901
- Frontiers in immunology 13:10786782022Pro-resolving and anti-arthritic properties of the MC selective agonist PL8177.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose Garrido-Mesa,Bethan Lynne Thomas,John Dodd,Carl Spana,Mauro Perretti,Trinidad Montero-MelendezPubMed 36505403
- Frontiers in pharmacology 12:7201732022AKT-AMPKα-mTOR-dependent HIF-1α Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEslam E Abd El-Fattah,Sameh Saber,Mahmoud E Youssef,Hanan Eissa,Eman El-Ahwany,Noha A Amin,Mohammed Alqarni,Gaber El-Saber Batiha,Ahmad J Obaidullah,Mohamed M Y Kaddah,Ahmed Gaafar Ahmed Gaafar,Ahmed A E Mourad,Gomaa Mostafa-Hedeab,Amir Mohamed AbdelhamidPubMed 35095479
- Oral diseases 29:528-5412021Surfactin induces autophagy, apoptosis, and cell cycle arrest in human oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThi Thuy Tien Vo,Yinshen Wee,Hsin-Chung Cheng,Ching-Zong Wu,Yuh-Lien Chen,Vo Phuoc Tuan,Ju-Fang Liu,Wei-Ning Lin,I-Ta LeePubMed 34181793
- Frontiers in psychiatry 12:6780902021GABA Alpha 2,3 Modulation Improves Select Phenotypes in a Mouse Model of Fragile X Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTori L Schaefer,Amy A Ashworth,Durgesh Tiwari,Madison P Tomasek,Emma V Parkins,Angela R White,Andrew Snider,Matthew H Davenport,Lindsay M Grainger,Robert A Becker,Chandler K Robinson,Rishav Mukherjee,Michael T Williams,Jay R Gibson,Kimberly M Huber,Christina Gross,Craig A EricksonPubMed 34093287
- Frontiers in cellular neuroscience 14:1892020Potential Involvement of Adiponectin Signaling in Regulating Physical Exercise-Elicited Hippocampal Neurogenesis and Dendritic Morphology in Stressed Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPingjie Wang et. al.PubMed 32774242
- Molecular medicine reports 22:67-762020miR‑133b affects cell proliferation, invasion and chemosensitivity in renal cell carcinoma by inhibiting the ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Xu et. al.PubMed 32377748
- Oncology reports 41:2967-29742019Downregulation of estrogen receptor β inhibits lung adenocarcinoma cell growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjuan Chen et. al.PubMed 30864727
- International journal of molecular sciences 20:2018NFκB Inhibition Mitigates Serum Amyloid A-Induced Pro-Atherogenic Responses in Endothelial Cells and Leukocyte Adhesion and Adverse Changes to Endothelium Function in Isolated Aorta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbigail Vallejo et. al.PubMed 30597899
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 0.2 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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ERK1/2 (pT202/Y204) ELISA Kit is a single-wash 90-min Simplestep used to quantify ERK1/2 (pT202/Y204) with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Range
- 0.2 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 0.1 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample A431 Extract | n 6 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample A431 Extract | n 3 | C.V. 1.3 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ERK1/2 (pT202/Y204) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of ERK1/2 (pT202/Y204) protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERK1 and ERK2 also known as MAPK3 and MAPK1 respectively are protein kinases involved in the mitogen-activated protein kinase (MAPK) signaling pathway. They share high sequence identity and exhibit similar functions. ERK1 has a molecular weight of approximately 44 kDa while ERK2 weighs around 42 kDa. Both are expressed ubiquitously in various tissues playing roles in diverse cellular processes. These proteins often detected through ERK1/2 western blot analyses present similar ERK protein size and ERK molecular weight characteristics relevant during protein expression studies.
- Biological function summary
ERK1 and ERK2 play key roles in cell proliferation differentiation and survival. They form part of a cascade that includes upstream activators such as MEK1/2 and downstream targets including transcription factors. As components of the MAPK signaling complex ERK1/2 regulate gene expression through phosphorylation events impacting cellular responses to various stimuli. Their activation often hinges on growth factors cytokines and stress signals facilitating cellular adaptation to environmental changes.
- Pathways
Regarding pathways ERK1/2 sit within the MAPK/ERK pathway and are significant in the Ras/Raf/MEK/ERK cascade one of the foremost signaling mechanisms in cells. They interact with several proteins including Ras and Raf which modulate their activation. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus impacting gene regulation and cell fate decisions. ERK1/2 proteins therefore serve as critical nodes linking extracellular signals to cellular responses ensuring balanced cell function.
- Associated diseases and disorders
ERK1/2 play significant roles in cancer and neurodegenerative diseases. Their overactivation is often linked to oncogenic processes contributing to cell proliferation in cancers. Mutations or dysregulation within the MAPK/ERK pathway including ERK1/2 frequently associate with tumorigenesis. Additionally in neurodegenerative disorders like Alzheimer's disease altered ERK1/2 activity may impact neuronal survival and function often through interaction with amyloid-beta and tau proteins further illustrating their importance in disease pathophysiology.
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5 product images
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (ab176640)
Typical recombinant protein standard curve.
Example of a typical ERK1/2 (pT202/Y204) recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (ab176640)
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of ERK1/2 (pT202/Y204) are normalized and plotted.
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (ab176640)
Comparison of total ERK1/2 expression in different cell lines.
Cell line analysis for Total ERK1/2 from 100 μg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (ab176640)
ERK1/2 (pT202/Y204) phosphorylation in response to EGF treatment.
Induction of ERK1/2 (pT202/Y204) phosphorylation in MCF-7 cells in response to EGF treatment. MCF-7 cells were cultured in 96-well tissue culture plates, serum starved and treated (10 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of ERK1/2 (pT202/Y204) are plotted and compared against Total ERK1/2 protein levels. Comparative ERK1/2 (pT202/Y204) and ERK1/2 (Total) data also shown by Western Blot.
Sandwich ELISA - ERK1/2 (pT202/Y204) ELISA Kit (ab176640)
Typical cell lysate dilution series.
Example of a typical ERK1/2 (pT202/Y204) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
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