ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA Kit

Abcam's ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA kit is designed for the semi-quantitative measurement of ERK1/2 (pT202/Y204) and Total ERK1/2 protein in Human and mouse cells.

The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

ASSAY SPECIFICITY

The ERK1/2 (pT202/Y204) assay detects endogenous levels of ERK1/2 (GenBank Accessions NP 002737.2 [ERK1], NP 620407 [ERK2]) in cellular lysates, only when phosphorylated at Thr202/Tyr204.

The ERK1/2 Total assay detects endogenous levels of ERK1/2 (GenBank Accessions NP 002737.2 [ERK1], NP 620407 [ERK2]) in cellular lysates, irrespective of phosphorylation status.

SPECIES REACTIVITY

This kit detects ERK1/2 (pT202/Y204) and ERK1/2 Total in Human and mouse cell culture extracts. Detection in rat samples is also expected. Other species should be tested on a case-by-case basis.

Serum and plasma samples have not been tested with this kit.

As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.

Estimated sensitivity: Phospho-ERK (Thr202/Tyr204): 0.1 ng/mL (tested with recombinant protein), Total ERK1/2: 0.6 ng/mL (tested with recombinant protein)
Range: Phospho-ERK (Thr202/Tyr204): 0.2-20ng/mL, Total ERK1/2: 0.6-60 ng/mL

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.