AB126445

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit

Name: ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (ab126445) | Abcam
Brand: Abcam Limited
SKU: AB126445

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(7 Publications)

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit is a ELISA for the measurement of ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 in Human, Mouse, Rat in Cell/Tissue Extracts samples.

View Alternative Names

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK, ERK2, PRKM1, PRKM2, MAPK1, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2

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Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

sELISA

Supplier Data

Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.

sELISA

Supplier Data

Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126445.

Supplier Data

Western blot - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using Western Blot.

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse, Rat, Human

Results type

Semi-Quantitative

Assay time

Assay Platform

Microplate

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Reactivity data

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Product details

ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.

Biological function summary

ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.

Pathways

ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.

Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.

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Product protocols

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Phosphorylates GJA1 at 'Ser-279' and 'Ser-282' resulting in an increase in GJA1 ubiquitination and ultimately lysosomal degradation (By similarity).

See full target information MAPK3 phospho T202 + Y204

Additional targets

MAPK1 phospho T185 + Y187

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:9 PubMed39958883

2025

Hypoxia-induced S-phase kinase-interacting protein 2 knockdown repressed the progression of melanoma through extracellular signal-regulated kinase 1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Hu

International journal of rheumatic diseases 26:80-87 PubMed36195975

2022

Casticin promotes osteogenic differentiation of bone marrow stromal cells and improves osteoporosis in rats by regulating nuclear factor-κB/mitogen-activated protein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Xuejia Li,Jianmin Li,Wanxin Liu,Ying Yu,Shuqiang Wang,Xiaojian Ye

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:711-72 PubMed34118146

2021

FGF21 promotes wound healing of rat brain microvascular endothelial cells through facilitating TNF-α-mediated VEGFA and ERK1/2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weiting Chen,Zhongen Shen,Shuiqi Cai,Long Chen,Dabin Wang

Cell chemical biology 28:1169-1179.e6 PubMed33571455

2021

Fatty acid chain length drives lysophosphatidylserine-dependent immunological outputs.

Applications

Unspecified application

Species

Unspecified reactive species

Neha Khandelwal,Minhaj Shaikh,Amol Mhetre,Shubham Singh,Theja Sajeevan,Alaumy Joshi,Kithiganahalli Narayanaswamy Balaji,Harinath Chakrapani,Siddhesh S Kamat

Oxidative medicine and cellular longevity 2019:8218650 PubMed31772710

2019

Melatonin Reduces Androgen Production and Upregulates Heme Oxygenase-1 Expression in Granulosa Cells from PCOS Patients with Hypoestrogenia and Hyperandrogenia.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yu,Rong-Xiang Wang,Meng-Hui Li,Tie-Cheng Sun,Yi-Wen Zhou,Yuan-Yuan Li,Li-Hua Sun,Bao-Lu Zhang,Zheng-Xing Lian,Song-Guo Xue,Yi-Xun Liu,Shou-Long Deng

Nature communications 9:4685 PubMed30410062

2018

Bi-directional signaling by membrane-bound KitL induces proliferation and coordinates thymic endothelial cell and thymocyte expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Buono,Marie-Laëtitia Thézénas,Alessandro Ceroni,Roman Fischer,Claus Nerlov

Journal of cellular physiology 233:7092-7102 PubMed29633272

2018

Effects of FGF-23-mediated ERK/MAPK signaling pathway on parathyroid hormone secretion of parathyroid cells in rats with secondary hyperparathyroidism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jun Chen,Xiong Chen,Wen-Jun Wu,Qi Zhou,Xiao-Hua Gong,Bi-Min Shi

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ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit

Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

Western blot - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

Key facts

Detection method

Sample types

Reacts with

Results type

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

Product promise