ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 in Mouse, Rat, Human in Cell Lysate samples.
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- International journal of rheumatic diseases 26:80-872022Casticin promotes osteogenic differentiation of bone marrow stromal cells and improves osteoporosis in rats by regulating nuclear factor-κB/mitogen-activated protein kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Zhang,Xuejia Li,Jianmin Li,Wanxin Liu,Ying Yu,Shuqiang Wang,Xiaojian YePubMed 36195975
- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:711-722021FGF21 promotes wound healing of rat brain microvascular endothelial cells through facilitating TNF-α-mediated VEGFA and ERK1/2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiting Chen,Zhongen Shen,Shuiqi Cai,Long Chen,Dabin WangPubMed 34118146
- Cell chemical biology 28:1169-1179.e62021Fatty acid chain length drives lysophosphatidylserine-dependent immunological outputs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeha Khandelwal,Minhaj Shaikh,Amol Mhetre,Shubham Singh,Theja Sajeevan,Alaumy Joshi,Kithiganahalli Narayanaswamy Balaji,Harinath Chakrapani,Siddhesh S KamatPubMed 33571455
- Oxidative medicine and cellular longevity 2019:82186502019Melatonin Reduces Androgen Production and Upregulates Heme Oxygenase-1 Expression in Granulosa Cells from PCOS Patients with Hypoestrogenia and Hyperandrogenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Yu et. al.PubMed 31772710
- Nature communications 9:46852018Bi-directional signaling by membrane-bound KitL induces proliferation and coordinates thymic endothelial cell and thymocyte expansion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Buono et. al.PubMed 30410062
- Journal of cellular physiology 233:7092-71022018Effects of FGF-23-mediated ERK/MAPK signaling pathway on parathyroid hormone secretion of parathyroid cells in rats with secondary hyperparathyroidism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jun Chen,Xiong Chen,Wen-Jun Wu,Qi Zhou,Xiao-Hua Gong,Bi-Min ShiPubMed 29633272
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
- Assay time
- 5h
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed:35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Additional Targets
MAPK1 phospho T185 + Y187
Alternative names
ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK
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ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 in Mouse, Rat, Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
- Assay time
- 5h
- Assay Platform
- Microplate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.
- Biological function summary
ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.
- Pathways
ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.
- Associated diseases and disorders
Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.
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3 product images
Western blot - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (ab126445)
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using Western Blot.
Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (ab126445)
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126445.
Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (ab126445)
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.
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