JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279794

ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit is a ELISA for the measurement of ERK2 (phospho T185 + Y187) + Total ERK2 in Human in Cell/Tissue Extracts samples.
3 Images
Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)
  • sELISA

Supplier Data

Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)

NIH/3T3 cells treated with/without PDGF. NIH/3T3 cells were untreated or treated with 50 ng/ml PDGFBB for 10 mins.

Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)
  • sELISA

Supplier Data

Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)

Positive Control.

NIH/3T3 cells were treated with PDGFBB at 37°C for 20 min.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)
  • sELISA

Supplier Data

Sandwich ELISA - ERK2 (phospho T185 + Y187) + Total ERK2 ELISA Kit (AB279794)

NIH/3T3 cells treated with/without PDGF. NIH/3T3 cells were untreated or treated with 50 ng/ml PDGFBB for 10 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB279855

Phospho-ERK1/ERK2/JNK1/P38 ELISA Kit

Sandwich ELISA - Phospho-ERK1/ERK2/JNK1/P38 ELISA Kit (AB279855)

  • 0
  • 0
Assay Kits

AB126445

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit

Sandwich ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ERK1/2 ELISA Kit (AB126445)

  • 0
  • 0
Assay Kits

AB126423

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA Kit

In-Cell ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) In-Cell ELISA Kit (AB126423)

  • 0
  • 0
Assay Kits

AB279793

ERK2 (phospho T185 + Y187) ELISA Kit

Sandwich ELISA - ERK2 (phospho T185 + Y187) ELISA Kit (AB279793)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-ERK2 (T185/Y187) and Total ERK2 (ab279794) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated ERK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-ERK2 and total ERK2. An anti-pan ERK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ERK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ERK2 (T185/Y187) antibody is added to detect phosphorylated ERK2. In the remaining wells, biotinylated anti-pan-ERK2 antibody is used to detect pan ERK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ERK2 (T185/Y187) or pan ERK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279794-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279794-CMP05", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279794-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279794-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279794-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279794-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279794-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-ERK2 detection antibody", "number":"AB279794-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ERK2 Coated Microplate", "number":"AB279794-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-ERK2 (T185/Y187)-antibody", "number":"AB279794-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279794-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - NIH/3T3 cell lysate", "number":"AB279794-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.
Biological function summary

ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.

Pathways

ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.

ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com