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AB272192

Exendin-4 ELISA Kit

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Exendin-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Exendin-4 with a sensitivity of 2.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Exendin-4

5 Images
ELISA - Exendin-4 ELISA Kit (AB272192)
  • ELISA

Supplier Data

ELISA - Exendin-4 ELISA Kit (AB272192)
Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
  • sELISA

Supplier Data

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)

Example of cell culture media Exendin-4 standard curve in Sample Diluent NS.

The Exendin-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
  • sELISA

Supplier Data

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)

Interpolated concentrations of spiked Exendin-4 RPMI-1640 cell culture media +/- 10% FBS samples.

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
  • sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)

Example of Exendin-4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
  • sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)

Recombinant Exendin-4 was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS in 96-well vs. 384-well plates. Undiluted samples are 25% cell culture media (RPMI containing 10% FBS). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media

Assay type

Sandwich (quantitative)

Sensitivity

= 2.58 pg/mL

Range

11.72 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Exendin-4 ELISA Kit ab272192 is a rapid single-wash 90-min Sandwich ELISA to measure Exendin-4 in cell culture media. This SimpleStep sensitivity is 2.58 pg/mL.

How the assay works

Exendin-4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Exendin-4 ELISA Kit ab272192 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "113 - 118 %", "average": "= 115" } ]
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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab272192-1x384Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272192-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272192-CMP08" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Capture Antibody", "number":"AB272192-CMP01" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Detector Antibody", "number":"AB272192-CMP02" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB272192-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB272192-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB272192-CMP07" }, { "size":"2 x 1 Vial", "name":"Exendin-4 Lyophilized Recombinant Protein", "number":"AB272192-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272192-CMP10" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272192-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272192-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272192-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272192-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272192-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272192-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272192-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272192-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Capture Antibody", "number":"AB272192-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Exendin-4 Lyophilized Recombinant Protein", "number":"AB272192-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Detector Antibody", "number":"AB272192-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272192-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exendin-4 also known as "exendine 4" or "exendin e 4" is a peptide with significant activity in glucose metabolism. The peptide originated from the saliva of the Gila monster Heloderma suspectum and has a molecular mass of approximately 4.2 kDa. It is expressed mainly in the pancreas but also found in other tissues. Exendin-4 acts as a potent agonist of the glucagon-like peptide-1 (GLP-1) receptor which is expressed in various parts of the body such as the brain and gastrointestinal tract.
Biological function summary

Exendin-4 enhances insulin secretion helping to regulate blood glucose levels effectively. It mimics the activity of GLP-1 which is involved in stimulating insulin release. Exendin-4 also slows gastric emptying and promotes satiety contributing to reduced food intake. Although the peptide does not form part of a larger complex its interactions directly affect multiple cellular processes relevant to metabolism.

Pathways

Exendin-4 participates in the insulin signaling and glucose homeostasis pathways. It influences insulin receptor substrate proteins thereby playing an important role in maintaining normal blood sugar levels. Exendin-4 modulates the activity of gluconeogenic enzymes connecting it to metabolic pathways involving energy balance and storage.

Exendin-4 shows relevance in treating type 2 diabetes and obesity. By enhancing insulin secretion and reducing appetite it provides therapeutic effects for individuals with diabetes. It shares functional characteristics with proteins like GLP-1 and insulin which are implicated in these metabolic disorders. Research continues to explore its potential benefits and application in managing these conditions more effectively.
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Product protocols

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Target data

Venom protein that mimics the incretin hormone glucagon-like peptide 1 (GLP-1). It stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. It also promotes satiety, reduces food intake, reduces fat deposition, reduces body weight and inhibits gastric emptying. Interacts with GLP-1 receptor (GLP1R). Induces hypotension that is mediated by relaxation of cardiac smooth muscle.
See full target information Exendin-4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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