AB325624

Exendin-4 ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
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Exendin-4 ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Exendin-4 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 0.91 - 2000 pg/ml with a sensitivity of 0.58 pg/mL
- Patent pending

View Alternative Names

Exendin-4

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sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit, Chemiluminescent (AB325624)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit, Chemiluminescent (AB325624)

Standard curve comparison between the Chemiluminescent Exendin-4 SimpleStep ELISA and colorimetric Exendin-4 SimpleStep ELISA (ab272192) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit, Chemiluminescent (AB325624)

Dynamic range comparison between the Chemiluminescent Exendin-4 SimpleStep ELISA, colorimetric Exendin-4 SimpleStep ELISA (ab272192), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

sELISA

Lab

Sandwich ELISA - Exendin-4 ELISA Kit, Chemiluminescent (AB325624)

Recombinant Exendin-4 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% RPMI and 25% RPMI + 10% fetal bovine serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.58 pg/mL

Range

0.91 - 2000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Exendin-4 ELISA Kit, Chemiluminescent (ab325624) is a rapid single-wash sandwich 55 min ELISA to measure Exendin-4 in Cell Culture Supernatant with a sensitivity of 0.58 pg/mL.

How the assay works

Exendin-4 ELISA Kit, Chemiluminescent (ab325624) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Exendin-4 (ab325624) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.9" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "113 - 118 %", "average": "= 115" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325624-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325624-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325624-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325624-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Detector Antibody", "number":"AB325624-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325624-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325624-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325624-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Exendin-4 Lyophilized Recombinant Protein", "number":"AB325624-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325624-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Exendin-4 Capture Antibody", "number":"AB325624-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Venom protein that mimics the incretin hormone glucagon-like peptide 1 (GLP-1). It stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. It also promotes satiety, reduces food intake, reduces fat deposition, reduces body weight and inhibits gastric emptying. Interacts with GLP-1 receptor (GLP1R). Induces hypotension that is mediated by relaxation of cardiac smooth muscle.

See full target information Exendin-4

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com