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AB326321

Exosome ELISA kit (cell culture)

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Exosome ELISA kit (cell culture) (ab326321) is an assay based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity.
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Indirect ELISA - Exosome ELISA kit (cell culture) (AB326321)
  • I-ELISA

Supplier Data

Indirect ELISA - Exosome ELISA kit (cell culture) (AB326321)

Example of linear range with the exosome standard of serum for ab326321.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "I-ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Exosome ELISA kit (cell culture) (ab326321) is an assay based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As one of the components (capture antibody) is coating a support (immunosorbent) the antigen antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate.

After incubation with the sample and subsequent washing, an antigen-specific HRP-conjugated antibody is added. After an intermediate wash to remove any excess antibody, a colourimetric reaction mediated by the HRP enzyme is observed with the naked eye when the substrate solution (TMB) is added. This reaction can be quantified using a spectrophotometer or colorimeter.

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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab326321-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"H2SO4 0,5M: Stop solution", "number":"AB326321-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Immunoplate 96-well (coated with CD63)", "number":"AB326321-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Standard for assay calibration: Lyophilized PC3 Exosome Standards", "number":"AB326321-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Washing buffer 25X", "number":"AB326321-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Buffer diluent 1X", "number":"AB326321-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"Primary Antibody", "number":"AB326321-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin conjugated HRP-Conjugated antibody", "number":"AB326321-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB", "number":"AB326321-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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