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Feline IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-10 in Cat in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cat

Range

0.205 - 50 ng/mL

Sensitivity

= 0.2 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
500X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Anti-Feline IL-10 coated Microplate (12 x 8 wells)
1 x 1 Unit
Biotinylated Anti-Feline IL-10 Antibody
2 x 1 Vial
Feline IL-10 Standard (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB Substrate Solution
1 x 12 mL

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Feline IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-10 in Cat in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cat

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.2 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 82.07

Range

71 - 100 %

Sample type

Plasma

Average %

= 86.6

Range

73 - 104 %

Sample type

Cell culture media

Average %

= 99.06

Range

87 - 111 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Feline IL-10 ELISA Kit (ab273228) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-10 in serum (feline IL-10 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for feline IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- feline IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Feline IL-10 ELISA Kit (ab273228), expandable thumbnail

    Sandwich ELISA - Feline IL-10 ELISA Kit (ab273228)

    This standard curve is for demonstration only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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