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Feline IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-10 in Cat in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Cat
0.205 - 50 ng/mL
= 0.2 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Feline IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-10 in Cat in Plasma, Cell culture supernatant, Serum samples.
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Cat
0.205 - 50 ng/mL
Pre-coated microplate (12 x 8 well strips)
= 0.2 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 82.07 | Range 71 - 100 % |
Sample type Plasma | Average % = 86.6 | Range 73 - 104 % |
Sample type Cell culture media | Average % = 99.06 | Range 87 - 111 % |
Blue Ice
-20°C
-20°C
-20°C
Feline IL-10 ELISA Kit (ab273228) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-10 in serum (feline IL-10 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for feline IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- feline IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Full details and terms and conditions can be found here:
Terms & Conditions.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com