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AB273230

Feline IL-1beta ELISA Kit

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Feline IL-1beta ELISA Kit is a Quantitative ELISA for the measurement of Feline IL-1beta in Cat in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, IL1B

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ELISA - Feline IL-1beta ELISA Kit (AB273230)
  • ELISA

Supplier Data

ELISA - Feline IL-1beta ELISA Kit (AB273230)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cat

Assay type

Quantitative

Sensitivity

= 16 pg/mL

Range

16 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Feline IL-1 beta ELISA Kit (ab273230) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-1 beta in serum, plasma and cell culture supernatants (feline IL-1 beta concentration is low in normal serum and plasma samples, and may not be detectable in this assay).

This assay employs an antibody specific for feline IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- feline IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 102 %", "average": "= 95.87" }, { "sample": "Plasma", "range": "86 - 102 %", "average": "= 94.84" }, { "sample": "Cell culture media", "range": "96 - 117 %", "average": "= 105.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273230-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273230-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273230-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Feline IL-1beta Antibody", "number":"AB273230-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273230-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Feline IL-1beta Standard (Lyophilized)", "number":"AB273230-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273230-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB273230-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Feline IL-1beta coated Microplate (12 x 8 wells)", "number":"AB273230-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information IL1B
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