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AB273226

Feline IL-4 ELISA Kit

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Feline IL-4 ELISA Kit is a Sandwich ELISA for the measurement of Feline IL-4 in Cat in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

1 Images
Sandwich ELISA - Feline IL-4 ELISA Kit (AB273226)
  • sELISA

Supplier Data

Sandwich ELISA - Feline IL-4 ELISA Kit (AB273226)

Feline IL-4 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.3 ng/mL

Range

0.33 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Feline IL-4 ELISA Kit (ab273226) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Feline IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Feline IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Feline IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 143 %", "average": "= 126.7" }, { "sample": "Plasma", "range": "107 - 124 %", "average": "= 115.2" }, { "sample": "Cell culture media", "range": "77 - 91 %", "average": "= 86.89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273226-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273226-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273226-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Feline IL-4 coated Microplate (12 x 8 wells)", "number":"AB273226-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273226-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273226-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Feline IL-4 Standard (Lyophilized)", "number":"AB273226-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Feline IL-4 Antibody", "number":"AB273226-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273226-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4
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websiteProtocolBooklet
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Product promise

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