Feline IL-4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-4 in Cat in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cat
- Range
- 0.33 - 80 ng/mL
- Sensitivity
- = 0.3 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Alternative names
Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4
What's included?
Recommended products
Feline IL-4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-4 in Cat in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cat
- Range
- 0.33 - 80 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.3 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 126.7 | Range 109 - 143 % |
Sample type Plasma | Average % = 115.2 | Range 107 - 124 % |
Sample type Cell culture media | Average % = 86.89 | Range 77 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Feline IL-4 ELISA Kit (ab273226) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Feline IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Feline IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Feline IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Feline IL-4 ELISA Kit (ab273226)
Feline IL-4 ELISA Kit standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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