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Feline IL-8 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-8 in Cat in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cat

Range

25 - 6000 pg/mL

Sensitivity

= 25 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Biotinylated feline IL-8 detection antibody
2 x 1 Vial
Pre-coated feline IL-8 Microplate (12 strips x 8 wells)
1 x 1 Unit
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL
feline IL-8 Standards (lyophilized)
2 x 1 Vial

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Feline IL-8 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-8 in Cat in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Cat

Range

25 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 25 pg/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 89.71

Range

82 - 97 %

Sample type

Plasma

Average %

= 84.6

Range

71 - 95 %

Sample type

Cell culture media

Average %

= 118

Range

100 - 149 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's IL-8 Feline ELISA Kit (ab193688) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-8 in serum, plasma (feline IL8 concentration is pretty low in normal plasma, it may not be detected in this assay) and cell culture supernatant.

This assay employs an antibody specific for feline IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-8 present in the samples. The wells are washed and biotinylated anti-feline IL8 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-8 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Supplementary info

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Activity summary

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

Associated diseases and disorders

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Feline IL-8 ELISA Kit (ab193688), expandable thumbnail

    Sandwich ELISA - Feline IL-8 ELISA Kit (ab193688)

    Standard Curve using ab193688 IL-8 Feline ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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