Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Feline IL-8 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-8 in Cat in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Cat
25 - 6000 pg/mL
= 25 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, IL8, CXCL8
Feline IL-8 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Feline IL-8 in Cat in Plasma, Cell culture supernatant, Serum samples.
Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, IL8, CXCL8
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Cat
25 - 6000 pg/mL
Pre-coated microplate (12 x 8 well strips)
= 25 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 89.71 | Range 82 - 97 % |
Sample type Plasma | Average % = 84.6 | Range 71 - 95 % |
Sample type Cell culture media | Average % = 118 | Range 100 - 149 % |
Blue Ice
-20°C
-20°C
-20°C
Abcam's IL-8 Feline ELISA Kit (ab193688) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-8 in serum, plasma (feline IL8 concentration is pretty low in normal plasma, it may not be detected in this assay) and cell culture supernatant.
This assay employs an antibody specific for feline IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-8 present in the samples. The wells are washed and biotinylated anti-feline IL8 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-8 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.
IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.
IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Standard Curve using ab193688 IL-8 Feline ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com