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AB273233

Feline Resistin ELISA Kit

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Feline Resistin ELISA Kit is a Sandwich ELISA for the measurement of Feline Resistin in Cat in Biofluids, Cell Culture Media samples.
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ELISA - Feline Resistin ELISA Kit (AB273233)
  • ELISA

Supplier Data

ELISA - Feline Resistin ELISA Kit (AB273233)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Cat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.5 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Feline resistin ELISA Kit (ab273233) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline resistin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for feline resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-feline resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision & recovery information

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

Recovery

[ { "sample": "Serum", "range": "82 - 106 %", "average": "= 91.57" }, { "sample": "Plasma", "range": "93 - 142 %", "average": "= 113.9" }, { "sample": "Cell culture media", "range": "83 - 147 %", "average": "= 106.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273233-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273233-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273233-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273233-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273233-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Feline Resistin coated Microplate (12 x 8 wells)", "number":"AB273233-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Feline Resistin Standard (Lyophilized)", "number":"AB273233-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Feline Resistin Antibody", "number":"AB273233-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB273233-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
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Product protocols

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Target data

See full target information Resistin
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websiteProtocolBooklet
en
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Product promise

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