AB324919

Free BDNF ELISA Kit - Extracellular

SimpleStep
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Free BDNF ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Free BDNF with a sensitivity of 1.751pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Brain-derived neurotrophic factor, Bdnf

9 Images

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Free BDNF concentration was determined to be 336.0 pg/mL with a range of 127.3 – 812.5 pg/mL.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Interpolated concentration of native Free BDNF was measured in duplicate at different sample concentrations. Undiluted samples are 50% unstimulated L929 supernatant, and stimulated L929 supernatant (1.5% PHA + 10 ng/ml PMA for 72 hours). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Example of Free BDNF standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Recombinant prepro-BDNF, pro-BDNF, and mature BDNF proteins were tested for reactivity in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed. The kit reacts with both free mature and pro-BDNF but weakly detects prepro-BDNF.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Interpolated concentration of native Free BDNF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are as follows : human serum 5%. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Interpolated concentration of native Free BDNF was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 5%, human plasma (citrate) 10%, human plasma (EDTA) 6.25%, human plasma (heparin) 12.5%, and rat serum 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Standard curve comparison between the original BDNF SimpleStep ELISA (ab212166), the leading competitor kit, and the current Free BDNF SimpleStep ELISA. The new kit ab324919 shows increased sensitivity.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Human, mouse, rat, bovine and monkey serum was assayed at 2.5% in duplicate. Interpolated concentration of native Free BDNF was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Free BDNF ELISA Kit - Extracellular (AB324919)

Example of Free BDNF standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Supernatant, Urine, Cerebral Spinal Fluid

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.751 pg/mL

Range

5.625 - 360 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Free BDNF ELISA Kit- Extracellular (ab324919) is a rapid single-wash sandwich 90-min ELISA to measure Free BDNF in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Supernatant, Urine, Cerebrospinal Fluid. This SimpleStep sensitivity is 1.751 pg/ml.

How the assay works

Free BDNF ELISA Kit- Extracellular (ab324919) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Free BDNF (ab324919) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Free BDNF ELISA Kit- Extracellular only requires 12.5µL sample.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 109 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "78 - 83 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "98 - 114 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "88 - 95 %", "average": "= 92" }, { "sample": "Cell culture supernatant", "range": "101 - 111 %", "average": "= 104" }, { "sample": "Urine", "range": "78 - 83 %", "average": "= 80" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 105 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity).. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.

See full target information Bdnf

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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