AB322084

Gentamicin ELISA Kit - Extracellular

SimpleStep
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Gentamicin ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Gentamicin with a sensitivity of 55.11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

Gentamycin

4 Images

Competitive ELISA - Gentamicin ELISA Kit - Extracellular (AB322084)

cELISA

Supplier Data

Competitive ELISA - Gentamicin ELISA Kit - Extracellular (AB322084)

Gentamicin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent SSW. Undiluted samples are 10% Human serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - Gentamicin ELISA Kit - Extracellular (AB322084)

Gentamicin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent SSW. Undiluted samples are as follows : 10% Human Urine, 10% Human Breast Milk, and 25% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - Gentamicin ELISA Kit - Extracellular (AB322084)

Gentamicin was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent SSW. Undiluted samples are 10% Mouse serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - Gentamicin ELISA Kit - Extracellular (AB322084)

Example of Gentamicin standard curve. OD (450 nm) values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Serum, Milk, EDTA Plasma, Citrate plasma, Cell culture media

Assay type

Competitive

Results type

Quantitative

Sensitivity

= 55.11 pg/mL

Range

62.5 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Gentamicin SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Gentamicin in Serum, Cit Plasma, EDTA Plasma, Urine, Cell culture media, and Milk. Quantitate Gentamicin with 55.11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]

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Recovery

[ { "sample": "Serum", "range": "112 - 118 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "109 - 115 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "107 - 110 %", "average": "= 108" }, { "sample": "Urine", "range": "86 - 90 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "105 - 110 %", "average": "= 107" }, { "sample": "Milk", "range": "97 - 100 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gentamicin is not a typical biological target but rather an aminoglycoside antibiotic used in targeting bacteria. It does not possess a protein structure with a mass as it is a compound. Gentamicin acts mechanically by binding to the 30S subunit of bacterial ribosomes causing a misreading of mRNA and disrupting protein synthesis. It mainly targets gram-negative bacteria but also shows activity against some gram-positive bacteria. Gentamicin is typically administered in settings like hospitals for treating severe infections.

Biological function summary

The main activity of this antibiotic disrupts the normal function of the bacterial ribosome. It is not a part of a complex but interacts with the ribosome complex consisting of ribosomal RNA and various ribosomal proteins. By binding to the 30S subunit gentamicin causes errors in the translation process preventing the bacteria from synthesizing necessary proteins needed for growth and proliferation.

Pathways

Disruptions caused by gentamicin impact the bacterial translation pathway significantly. It affects the elongation cycle of protein synthesis where aminoacyl-tRNA enters the ribosomal A site. The faulty incorporation of amino acids due to gentamicin's effect hinders the elongation pathway leading to nonfunctional proteins and bacterial death. This process relates components like other aminoglycoside antibiotics such as amikacin which function similarly.

Severe bacterial infections often require gentamicin intervention. It is particularly relevant in treating infections caused by Pseudomonas aeruginosa and those leading to conditions such as sepsis and complicated urinary tract infections. Proteins involved in bacterial virulence and resistance such as efflux pumps are associated with how certain bacteria may resist gentamicin action impacting its effectiveness in these diseases.

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Product protocols

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Target data

See full target information Gentamicin

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websiteProtocolBooklet

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Product promise

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