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AB171581

GFP ELISA Kit

5

(3 Reviews)

|

(23 Publications)

GFP ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantify Green Fluorescence protein with a sensitivity of 2.8 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility – works with tissue lysate and cell lysate.
- Cited in over 10 publications

See more GFP assay kits and antibodies

View Alternative Names

Green fluorescent protein, GFP

10 Images
Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Lab

Sandwich ELISA - GFP ELISA Kit (AB171581)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Unknown

Sandwich ELISA - GFP ELISA Kit (AB171581)

Linearity of dilution for spiked GFP.

GFP spiked 143B lysate was measured in 3-fold dilution series

ELISA - GFP ELISA Kit (AB171581)
  • ELISA

Supplier Data

ELISA - GFP ELISA Kit (AB171581)

Example GFP standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Unknown

Sandwich ELISA - GFP ELISA Kit (AB171581)

Example of raw data for GFP standard curve.

Raw data values are shown in the table

Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit (AB171581)

"Representative image of GFP ELISA Kit (ab171581)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X GFP Capture Antibody

- 10X GFP Detector Antibody

- GFP Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent EB

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

"

ELISA - GFP ELISA Kit (AB171581)
  • ELISA

Supplier Data

ELISA - GFP ELISA Kit (AB171581)

Titration of GFP spiked 143B cell lysate within the working range of the assay.

Background subtracted data from triplicate measurements are plotted.

ELISA - GFP ELISA Kit (AB171581)
  • ELISA

AbReview

ELISA - GFP ELISA Kit (AB171581)

The kit works with superfold GFP. See Abreview.

This image is courtesy submitted by Ling Chun

ELISA - GFP ELISA Kit (AB171581)
  • ELISA

Lab

ELISA - GFP ELISA Kit (AB171581)

GFP cross reactivity data.

This kit recognizes both GFP and enhanced GFP (eGFP) in cell and tissue extracts. No reactivity with mCherry, RFP or YFP was observed.

Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit (AB171581)

Recombinant GFP was spiked into the following human samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR in 96-well and 384-well plates. Undiluted samples are 125 μg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit (AB171581)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit (AB171581)

Example of GFP standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Sandwich (quantitative)

Sensitivity

= 2.8 pg/mL

Range

18.8 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GFP ELISA Kit ab171581 is a rapid single-wash 90-min ELISA kit to measure GFP (Green Fluorescence Protein) in tissue lysates and cell lysates. This SimpleStep ELISA® sensitivity is 2.8 pg/mL.

How the assay works

GFP (Green Fluorescence Protein) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit has been validated to detect both the Enhanced and Superfold variants of GFP.

GFP ELISA Kit ab171581 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using GFP ELISA Kit ab171581:

GFP ELISA Kit ab171581 has been used to visualize tauopathy in Alzheimer's disease (1), to study adeno-associated viral vectors for human gene transfer (2), and to analyze protein secretions from Parkinson's Disease patients (3).

References:

(1)Marcia N Gordon et al. 2023, PMID: 36970910,
(2)Catherine O'Riordan et al. 2020, PMID: 32578442,
(3)Bruno Manadas et al. 2020, PMID: 32266064

Save your precious samples

Our 384-well plate GFP ELISA Kit only requires a maximum of 12.5µL

Related and recommended products

GFP ELISA Kit ab171581 is commonly used to study gene expression and protein interactions. GFP is a fluroscent protein used for labeling and visualizing cellular processes.

Other ELISA kits for labeling and visualization include:

- mCherry ELISA kit ab221829

Secondary antibodies for labeling and visualization include:

- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Lysate", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Intra", "sample": "Cell Lysate", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Fetal Bovine Serum", "range": "96 - 98 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GFP also known as Green Fluorescent Protein acts as a bioluminescent marker derived from the jellyfish Aequorea victoria. GFP is popular in molecular biology for its fluorescence properties making it useful for visualizing proteins. This protein has a molecular weight of approximately 27 kDa. Researchers and scientists often express GFP in various organisms as a luminescent tag helping them observe protein expression localization and interaction within cells. GFP tagging involves the fusion of GFP to a protein of interest enabling the study of the protein's function and dynamics without affecting the host cell.
Biological function summary

GFP serves as a marker due to its ability to emit green fluorescence without requiring additional substrates or cofactors. GFP does not function within complexes like other proteins but acts as a standalone tool to monitor physiological processes. Scientists utilize techniques such as Western blot ELISA and microscopy along with GFP to track and quantify proteins inside living cells. Anti-GFP antibodies can detect GFP fusion proteins in various applications providing valuable insights into protein behavior and allowing robust assays involving GFP.

Pathways

GFP itself does not participate actively in traditional biochemical or signaling pathways. Instead it enables visual tracking within pathways. Researchers utilize GFP to study pathways like MAPK/ERK and PI3K/AKT where they track proteins related to these pathways using GFP tagging. For instance fusing GFP with proteins like ERK1/2 allows tracking phosphorylation events and signal transduction in living cells leading to better understanding of cellular responses to different stimuli.

Researchers use GFP as a model to study gene expression and protein interactions under disease conditions. For example in neurological disorders GFP helps visualize neuronal pathways and protein aggregation processes. By tagging proteins such as amyloid precursor protein (APP) or tau with GFP scientists can study their role in Alzheimer's disease progression. Similarly GFP facilitates the investigation of cancer pathways allowing visualization of tumor-related proteins and helping researchers study how cancer cells grow and invade tissues supporting cancer research and therapy development.
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Product protocols

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4007 PubMed37414857

2023

Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Ralf Nilsson,Elisa Lázaro-Ibáñez,Hanna Duàn,Tasso Miliotis,Marie Strimfors,Michael Lerche,Ana Rita Salgado Ribeiro,Johan Ulander,Daniel Lindén,Anna Salvati,Alan Sabirsh

Journal of Alzheimer's disease : JAD 93:365-378 PubMed36970910

2023

Influence of Host Age on Intracranial AAV9 TauP301L Induced Tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Amber M Tetlow,Brianna M Jackman,Mohammed M Alhadidy,Varshini Perumal,David G Morgan,Marcia N Gordon

Poultry science 102:102247 PubMed36335737

2022

Sequential verification of exogenous protein production in OVA gene-targeted chicken bioreactors.

Applications

Unspecified application

Species

Unspecified reactive species

Young Min Kim,Ji Hyeon Shim,Jin Se Park,Hee Jung Choi,Kyung Min Jung,Kyung Youn Lee,Kyung Je Park,Jae Yong Han

Molecular therapy oncolytics 25:43-56 PubMed35399606

2022

Development of a low-seroprevalence, αvβ6 integrin-selective virotherapy based on human adenovirus type 10.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A Bates,James A Davies,Jana Váňová,Davor Nestić,Valerie S Meniel,Sarah Koushyar,Tabitha G Cunliffe,Rosie M Mundy,Elise Moses,Hanni K Uusi-Kerttula,Alexander T Baker,David K Cole,Dragomira Majhen,Pierre J Rizkallah,Toby Phesse,John D Chester,Alan L Parker

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35046019

2022

Tuning protein half-life in mouse using sequence-defined biopolymers functionalized with lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Koen Vanderschuren,Pol Arranz-Gibert,Minsoo Khang,Dagan Hadar,Alice Gaudin,Fan Yang,Ewa Folta-Stogniew,W Mark Saltzman,Miriam Amiram,Farren J Isaacs

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34686600

2021

An ELISA-based method for rapid genetic screens in .

Applications

Unspecified application

Species

Unspecified reactive species

Taylor R Jay,Yunsik Kang,Amanda Jefferson,Marc R Freeman

ACS pharmacology & translational science 4:1235-1245 PubMed34151213

2021

Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.

Applications

Unspecified application

Species

Unspecified reactive species

Paweł Kozielewicz,Rawan Shekhani,Stefanie Moser,Carl-Fredrik Bowin,Janine Wesslowski,Gary Davidson,Gunnar Schulte

Gene therapy : PubMed33542456

2021

Proteosomal degradation impairs transcytosis of AAV vectors from suprachoroidal space to retina.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Ding,Jikui Shen,Sean Hackett,Mahmood Khan,Peter A Campochiaro

Molecular therapy : the journal of the American Society of Gene Therapy 29:1729-1743 PubMed33484965

2021

A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Dooley,Russell E McConnell,Ke Xu,Nuruddeen D Lewis,Sonya Haupt,Madeleine R Youniss,Shelly Martin,Chang Ling Sia,Christine McCoy,Raymond J Moniz,Olga Burenkova,Jorge Sanchez-Salazar,Su Chul Jang,Bryan Choi,Rane A Harrison,Damian Houde,Dalia Burzyn,Charan Leng,Katherine Kirwin,Nikki L Ross,Jonathan D Finn,Leonid Gaidukov,Kyriakos D Economides,Scott Estes,James E Thornton,John D Kulman,Sriram Sathyanarayanan,Douglas E Williams

Scientific reports 11:708 PubMed33436911

2021

Transfer of oral bacteria to the fetus during late gestation.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Yu,Michelle Rodriguez,Zubin Paul,Elizabeth Gordon,Tongjun Gu,Kelly Rice,Eric W Triplett,Maureen Keller-Wood,Charles E Wood
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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