Name: GFP ELISA Kit
SKU: ab171581
Rating: 5 (3 reviews)

GFP ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantify Green Fluorescence protein with a sensitivity of 2.8 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility – works with tissue lysate and cell lysate.
- Cited in over 10 publications

Images

Sandwich ELISA - GFP ELISA Kit (AB171581), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Tissue Lysate, Cell Lysate
Assay type: Sandwich (quantitative)
Range: 18.8 - 1200 pg/mL
Assay time: 1h 30m
Sensitivity: = 2.8 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

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Target data

See full target information GFP

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

What's included?

1 x 384 Tests

Components

10X GFP Capture Antibody

1 x 600 µL

10X GFP Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

4X Antibody Diluent EB

1 x 6 mL

50X Cell Extraction Enhancer Solution (ab193971)

6 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

GFP Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Tissue Lysate, Cell Lysate
Assay type: Sandwich (quantitative)
Range: 18.8 - 1200 pg/mL
Assay time: 1h 30m
Assay Platform: Microplate
Sensitivity: = 2.8 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Cell Lysate	n 9	C.V. 3.1

Inter assay

Sample	n	C.V.
Sample Cell Lysate	n 3	C.V. 8.2

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Fetal Bovine Serum	Average % = 97	Range 96 - 98 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

GFP ELISA Kit ab171581 is a rapid single-wash 90-min ELISA kit to measure GFP (Green Fluorescence Protein) in tissue lysates and cell lysates. This SimpleStep ELISA® sensitivity is 2.8 pg/mL.

How the assay works

GFP (Green Fluorescence Protein) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit has been validated to detect both the Enhanced and Superfold variants of GFP.

GFP ELISA Kit ab171581 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using GFP ELISA Kit ab171581:

GFP ELISA Kit ab171581 has been used to visualize tauopathy in Alzheimer's disease ⁽¹⁾, to study adeno-associated viral vectors for human gene transfer ⁽²⁾, and to analyze protein secretions from Parkinson's Disease patients ⁽³⁾.

References:

⁽¹⁾Marcia N Gordon et al. 2023, PMID: 36970910,
⁽²⁾Catherine O'Riordan et al. 2020, PMID: 32578442,
⁽³⁾Bruno Manadas et al. 2020, PMID: 32266064

Save your precious samples

Our 384-well plate GFP ELISA Kit only requires a maximum of 12.5µL

Related and recommended products

GFP ELISA Kit ab171581 is commonly used to study gene expression and protein interactions. GFP is a fluroscent protein used for labeling and visualizing cellular processes.

Other ELISA kits for labeling and visualization include:

- mCherry ELISA kit mCherry ELISA Kit ab221829

Secondary antibodies for labeling and visualization include:

- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113

Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Sandwich ELISA - GFP ELISA Kit (ab171581)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
ELISA - GFP ELISA Kit (ab171581)
Example GFP standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - GFP ELISA Kit (ab171581)
Example of raw data for GFP standard curve.
Raw data values are shown in the table
ELISA - GFP ELISA Kit (ab171581)
GFP cross reactivity data.
This kit recognizes both GFP and enhanced GFP (eGFP) in cell and tissue extracts. No reactivity with mCherry, RFP or YFP was observed.
ELISA - GFP ELISA Kit (ab171581)
Titration of GFP spiked 143B cell lysate within the working range of the assay.
Background subtracted data from triplicate measurements are plotted.
This image is courtesy submitted by Ling Chun
ELISA - GFP ELISA Kit (ab171581)
The kit works with superfold GFP. See Abreview.
Sandwich ELISA - GFP ELISA Kit (ab171581)
Linearity of dilution for spiked GFP.
GFP spiked 143B lysate was measured in 3-fold dilution series
Sandwich ELISA - GFP ELISA Kit (ab171581)
Recombinant GFP was spiked into the following human samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR in 96-well and 384-well plates. Undiluted samples are 125 μg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - GFP ELISA Kit (ab171581)
Example of GFP standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.