JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326732

GFP ELISA Kit, Chemiluminescent - Intracellular

Be the first to review this product! Submit a review

|

(0 Publication)

GFP ELISA Kit, Chemiluminescent - Intracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of GFP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.82 - 1800 pg/ml with a sensitivity of 0.26 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Green fluorescent protein, GFP

4 Images
Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Lab

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Standard curve comparison between the Chemiluminescent GFP SimpleStep ELISA and colorimetric GFP SimpleStep ELISA (ab316916) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Lab

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Dynamic range comparison between the Chemiluminescent GFP SimpleStep ELISA and colorimetric GFP SimpleStep ELISA (ab316916). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Interpolated concentrations of spiked eGFP in Jurkat extract and spiked denatured-eGFP in Jurkat extract

Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1 : 20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Interpolated concentrations of GFP in HEK293 overexpressing GFP cell extract

Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Luminescent

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Aequorea victoria

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.26 pg/mL

Range

0.82 - 1800 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

GFP ELISA Kit, Chemiluminescent - Intracellular (ab326732) is a rapid single-wash sandwich 55-min ELISA to measure GFP in Tissue Extracts, Cell Lysate with a sensitivity of 0.26 pg/mL.

How the assay works

GFP ELISA Kit, Chemiluminescent - Intracellular (ab326732) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

GFP (ab326732) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GFP antibody [EPR28018-88] - BSA and Aside free (Capture) ab315128
- Anti-GFP antibody [EPR28018-89] - BSA and Aside free (Detector) ab315129
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.9" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "82 - 107 %", "average": "= 107" }, { "sample": "Cell Lysate", "range": "82 - 92 %", "average": "= 92" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326732-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326732-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326732-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326732-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GFP Lyophilized Recombinant Protein", "number":"AB326732-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326732-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Capture Antibody", "number":"AB326732-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326732-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Detector Antibody", "number":"AB326732-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326732-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB326732-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326732-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com