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AB316915

GFP ELISA Kit- Extracellular

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GFP ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify GFP with a sensitivity of 1.813 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

See more GFP assay kits and antibodies

View Alternative Names

Green fluorescent protein, GFP

3 Images
Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Recombinant untreated and 2% SDS-denatured eGFP were diluted 1 : 20 in 50% Hela cell culture supernatant (prepared in Sample Diluent NS) to prepare the undiluted sample, and then diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Standard curve comparison between the original GFP SimpleStep ELISA (ab171581) and current GFP SimpleStep ELISA (ab316915).

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Extracellular (AB316915)

Example of GFP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Aequorea victoria

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.813 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Cell culture supernatant. Quantitate  GFP with 1.813 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 104 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316915-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316915-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB316915-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316915-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316915-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316915-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Capture Antibody", "number":"AB316915-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316915-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB316915-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GFP Lyophilized Recombinant Protein", "number":"AB316915-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Detector Antibody", "number":"AB316915-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316915-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com