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GFP ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of GFP - Intracellular in Tissue Extracts, Cell Lysate samples.

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Images

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916), expandable thumbnail
  • Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916), expandable thumbnail
  • Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916), expandable thumbnail
  • Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Aequorea victoria

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Sensitivity

= 2.523 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

What's included?

1 x 96 Tests
Components
10X GFP Capture Antibody
1 x 600 µL
10X GFP Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent CPR2
1 x 6 mL
GFP Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

GFP ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of GFP - Intracellular in Tissue Extracts, Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Aequorea victoria

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 2.523 pg/mL

General recovery

93%

Precision

Intra assay

Sample

Extract

n

8

mean

-

SD

-

C.V.

6.1

Inter assay

Sample

Extract

n

3

mean

-

SD

-

C.V.

6.9

Recovery

Sample specific recovery

Sample type

Tissue Extracts

Average %

= 93

Range

82 - 107 %

Sample type

Cell Lysate

Average %

= 86

Range

82 - 92 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Tissue extracts, Cell Lysate. Quantitate  GFP with 2.523 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916), expandable thumbnail

    Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916)

    Example of GFP standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916), expandable thumbnail

    Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916)

    Standard curve comparison between the original GFP SimpleStep ELISA (GFP ELISA Kit ab171581) and current GFP SimpleStep ELISA (ab316916). 

  • Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916), expandable thumbnail

    Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916)

    Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.  

  • Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916), expandable thumbnail

    Sandwich ELISA - GFP ELISA Kit- Intracellular (ab316916)

    Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1:20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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