GFP ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of GFP - Intracellular in Tissue Extracts, Cell Lysate samples.
Colorimetric
Tissue Extracts, Cell Lysate
Sandwich (quantitative)
Aequorea victoria
9.375 - 600 pg/mL
1h 30m
= 2.523 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Green fluorescent protein, GFP
GFP ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of GFP - Intracellular in Tissue Extracts, Cell Lysate samples.
Colorimetric
Tissue Extracts, Cell Lysate
Sandwich (quantitative)
Aequorea victoria
9.375 - 600 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 2.523 pg/mL
93%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 6.1 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 6.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Tissue Extracts | Average % = 93 | Range 82 - 107 % |
Sample type Cell Lysate | Average % = 86 | Range 82 - 92 % |
Blue Ice
+4°C
+4°C
+4°C
GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Tissue extracts, Cell Lysate. Quantitate GFP with 2.523 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of GFP standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Standard curve comparison between the original GFP SimpleStep ELISA (GFP ELISA Kit ab171581) and current GFP SimpleStep ELISA (ab316916).
Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1:20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com