Guinea Pig Complement C3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Guinea Pig Complement C3 in Guinea pig in Serum samples.
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- ACS nano 16:10566-105802022PEGylated Polyester Nanoparticles Trigger Adverse Events in a Large Animal Model of Trauma and in Naı̈ve Animals: Understanding Cytokine and Cellular Correlations with These Events.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuzhat Maisha,Chhaya Kulkarni,Narendra Pandala,Rose Zilberberg,Leasha Schaub,Leslie Neidert,Jacob Glaser,Jeremy Cannon,Vandana Janeja,Erin B LavikPubMed 35822898
- ACS biomaterials science & engineering 6:4903-49152020Development of a Sensitive Assay to Screen Nanoparticles for Complement Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuzhat Maisha,Tobias Coombs,Erin LavikPubMed 33313396
- Arthritis research & therapy 21:1452019Calorie restriction with regular chow, but not a high-fat diet, delays onset of spontaneous osteoarthritis in the Hartley guinea pig model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren B Radakovich et. al.PubMed 31196172
- Bioconjugate chemistry 29:2436-24472018Engineering Intravenously Administered Nanoparticles to Reduce Infusion Reaction and Stop Bleeding in a Large Animal Model of Trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChimdiya Onwukwe et. al.PubMed 29965731
- Scientific reports 8:31182018Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaShawn A Hickman et. al.PubMed 29449604
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Guinea pig
- Range
- 3.13 - 3260 ng/mL
- Sensitivity
- = 1.3083 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.
Alternative names
Complement C3, C3
What's included?
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Guinea Pig Complement C3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Guinea Pig Complement C3 in Guinea pig in Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Guinea pig
- Range
- 3.13 - 3260 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 1.3083 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % > 85 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Complement C3 Guinea Pig ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Complement C3 levels in serum and blood .
In this assay the Complement C3 present in samples reacts with the anti-Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
- Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
- Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
- Associated diseases and disorders
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
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Sandwich ELISA - Guinea Pig Complement C3 ELISA Kit (ab157705)
Representative standard curve using ab157705 Complement C3 GuineaPig ELISA Kit.
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